LIF Protein (AA 23-202) (GST tag)

N° du produit ABIN1344370

Protéine Recombinant LIF exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour LIF Protein (AA 23-202) (GST tag) (ABIN1344370)

Antigène: LIF (Leukemia Inhibitory Factor (LIF))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain, Souris

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Pureté: >95 % (SDS-PAGE)

Détail du produit

Attributs du protein: AA 23-202

Purification/Conjugué: Cette LIF protéine est marqué à la GST tag.

Fonction: LIF (human) (rec.)

Specificité: Mouse and human embryonic stem cells.

Réactivité croisée: Humain, Souris

Attributs du produit: Human LIF (aa 23-202) is fused at the N-terminus to a tag.

niveau d'endotoxine: <0.05EU/μg purified protein (LAL test).

Biological Activity Comment: Maintains pluripotency of mouse and human embryonic stem cells. The LIF (GST-tagged) from AdipoGen has the same activity as cleaved LIF.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Concentration: Lot specific

Buffer: Contains PBS.

Conseil sur la manipulation: After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.

Stock: 4 °C,-20 °C

Stockage commentaire:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Date de péremption: 6 months

Détail du antigène

Antigène: LIF (Leukemia Inhibitory Factor (LIF))

Autre désignation: LIF

Sujet:

Leukemia Inhibitory Factor, Differentiation-stimulating Factor, D Factor, Melanoma-derived LPL Inhibitor

LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities also include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation and the stimulation of acute-phase protein synthesis in hepatocytes. LIF activates JAK & STAT signaling in human embryonic stem (ES) cells, but this pathway does not maintain pluripotency in these cells, which instead rely on FGF2-mediated ERK signaling. By contrast, mouse ES cells can be maintained by LIF-mediated JAK & STAT signaling. LIF binds to a high affinity heterodimeric receptor complex consisting of two proteins: LIF-R alpha that binds LIF with low affinity and the 130 kDa (gp130) subunit that by itself does not bind LIF, but is required for high affinity binding of LIF.

Poids moléculaire: ~46kDa (SDS-PAGE)

UniProt: P15018

Pathways: Signalistation JAK/STAT, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding