LIF Protein (AA 23-202)
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- Antigène Voir toutes LIF Protéines
- LIF (Leukemia Inhibitory Factor (LIF))
- Type de proteíne
- Recombinant
- Activité biologique
- Active
- Attributs du protein
- AA 23-202
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Origine
- Humain, Souris
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Source
- Escherichia coli (E. coli)
- Application
- SDS-PAGE (SDS)
- Specificité
- Mouse and human embryonic stem cells.
- Réactivité croisée
- Humain, Souris
- Attributs du produit
- Human LIF (aa 23-202) is fused at the N-terminus to a tag.
- Pureté
- >95 % (SDS-PAGE)
- niveau d'endotoxine
- <0.05EU/μg purified protein (LAL test, Lonza).
- Top Product
- Discover our top product LIF Protéine
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Leukemia Inhibitory Factor (LIF) (AA 23-202) protein
LIF Origine: Humain Hôte: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Leukemia Inhibitory Factor (LIF) (AA 24-203) (Active) proteinLIF Origine: Souris Hôte: Escherichia coli (E. coli) Recombinant > 95 % by SDS-PAGE and HPLC analyses. Active
Leukemia Inhibitory Factor (LIF) (AA 25-203) protein (Fc Tag)LIF Origine: Souris Hôte: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Leukemia Inhibitory Factor (LIF) (Active) proteinLIF Origine: Rat Hôte: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Leukemia Inhibitory Factor (LIF) (AA 24-203) protein (His tag)LIF Origine: Souris Hôte: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Leukemia Inhibitory Factor (LIF) (AA 23-202) protein (His tag)LIF Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant Greater than 90 % by SDS-PAGE gel analyses WB, ELISA
Leukemia Inhibitory Factor (LIF) (AA 23-202) protein (HIS-GST)LIF Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant > 90 % by SDS - PAGE SDS
Leukemia Inhibitory Factor (LIF) (AA 1-202) protein (GST tag)LIF Origine: Humain Hôte: Wheat germ Recombinant WB, ELISA, AP, AA
Leukemia Inhibitory Factor (LIF) proteinLIF Origine: Souris Hôte: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE
Leukemia Inhibitory Factor (LIF) (AA 23-202) (Active) protein (His tag)LIF Origine: Humain Hôte: Baculovirus infected Insect Cells Recombinant > 90 % by SDS - PAGE SDS Active
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Commentaires
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Maintains pluripotency of mouse and human embryonic stem cells. The LIF (GST-tagged) from AdipoGen has the same activity as cleaved LIF.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Concentration
- Lot specific
- Buffer
- Lyophilized. Contains PBS.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
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Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 6 months after receipt when stored at -20°C. - Date de péremption
- 6 months
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- Antigène
- LIF (Leukemia Inhibitory Factor (LIF))
- Autre désignation
- LIF (LIF Produits)
- Sujet
- LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities also include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation and the stimulation of acute-phase protein synthesis in hepatocytes. LIF activates JAK & STAT signaling in human embryonic stem (ES) cells, but this pathway does not maintain pluripotency in these cells, which instead rely on FGF2-mediated ERK signaling. By contrast, mouse ES cells can be maintained by LIF-mediated JAK & STAT signaling. LIF binds to a high affinity heterodimeric receptor complex consisting of two proteins: LIF-R alpha that binds LIF with low affinity and the 130 kDa (gp130) subunit that by itself does not bind LIF, but is required for high affinity binding of LIF.
- Poids moléculaire
- ~46kDa (SDS-PAGE)
- UniProt
- P15018
- Pathways
- Signalistation JAK/STAT, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding
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