TRAIL Protein (AA 99-291, Extracellular Domain, Soluble) (His tag)
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- Antigène Voir toutes TRAIL (TNFSF10) Protéines
- TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))
- Type de proteíne
- Recombinant
- Activité biologique
- Active
- Attributs du protein
- Soluble, Extracellular Domain, AA 99-291
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Origine
- Humain, Souris
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Source
- Escherichia coli (E. coli)
- Purification/Conjugué
- Cette TRAIL protéine est marqué à la His tag.
- Application
- SDS-PAGE (SDS)
- Marque
- SuperKillerTRAIL™
- Specificité
- Binds to mouse and less potently to human TRAIL receptors.
- Réactivité croisée
- Humain, Souris
- Attributs du produit
- The extracellular domain of mouse TRAIL (aa 99-291) is fused at the N-terminus to a His-tag and a linker peptide. The active multimeric conformation is stabilized by an inserted mutation allowing an additional CC-bridge.
- Pureté
- >95 % (SDS-PAGE)
- niveau d'endotoxine
- <0.01EU/μg purified protein (LAL test, Lonza).
- Top Product
- Discover our top product TNFSF10 Protéine
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Commentaires
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Induces apoptosis at concentrations of >10ng/ml as tested on human tumor cells. This effect can be efficiently blocked by recombinant mouse TRAIL receptors. This protein does not require a cross-linker (enhancer) for its potent biological activity.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- In 20 mM HEPES, pH 7.4, 300 mM NaCl, 0.01 % Tween 20, 1 % sucrose, 1 mM DTT.
- Agent conservateur
- Dithiothreitol (DTT)
- Précaution d'utilisation
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- -20 °C,-80 °C
- Stockage commentaire
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Short Term Storage: -20°C
Long Term Storage: -80°C
Once thawed it is recommended to prepare appropriate aliquots and to store them at -80°C. , Stable for at least 1 year after receipt when stored at -80°C. - Date de péremption
- 12 months
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- Antigène
- TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))
- Autre désignation
- TRAIL (TNFSF10 Produits)
- Synonymes
- APO2L Protein, Apo-2L Protein, CD253 Protein, TL2 Protein, TRAIL Protein, A330042I21Rik Protein, AI448571 Protein, APO-2L Protein, Ly81 Protein, Trail Protein, tl2 Protein, apo2l Protein, cd253 Protein, trail Protein, apo-2l Protein, trail1 Protein, xtrail1 Protein, tnfsf10l2 Protein, zgc:92320 Protein, TNLG6A Protein, TNF superfamily member 10 Protein, tumor necrosis factor (ligand) superfamily, member 10 Protein, tumor necrosis factor superfamily member 10 Protein, TNF superfamily member 10 S homeolog Protein, TNFSF10 Protein, Tnfsf10 Protein, tnfsf10.S Protein, tnfsf10 Protein
- Sujet
- TNF-related apoptosis-inducing ligand (TRAIL, Apo2L,CD253, TNFSF10) is a type II transmembrane protein of about 34 kDa. Like most members of the tumor necrosis factor (TNF) superfamily of cytokines TRAIL can be cleaved at the cell surface by metalloproteases to form a soluble molecule. Active TRAIL forms trimers and specifically binds to five distinct receptors: TRAIL-R1 (DR4, Apo2,CD261, TNFRSF10A), TRAIL-R2 (DR5, KILLER, TRICK2A,TRICK2B, CD262, TNFRSF10B), TRAIL-R3 (DcR1,LIT, TRID, CD263, TNFRSF10C), TRAIL-R4 (DcR2, TRUNDD, CD264, TNFRSF10D), and osteoprotegerin (OPG, OCIF, TNFRSF11B). Trimerized TRAIL triggers apoptosis upon ligation of cell surface TRAIL-R1 and/or TRAIL-R2 by inducing the formation of the so-called multiprotein death-inducing signaling complex (DISC).
- Poids moléculaire
- ~24kDa
- UniProt
- P50592
- Pathways
- Apoptose, Positive Regulation of Endopeptidase Activity
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