CDNF Protéine

N° du produit ABIN1405917

Détail du produit

Antigène: CDNF (Cerebral Dopamine Neurotrophic Factor (CDNF))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: CHO Cells

Application: Western Blotting (WB), SDS-PAGE (SDS), Cell Culture (CC), In vivo Studies (in vivo)

Attributs du produit: Biological properties: Protein is biologically active. Suitable for cell culture and in vivo testing. Biological activity of human CDNF was tested in rat 6-OHDA model of Parkinson’s disease and shown to be both neuroprotective and neurorestorative (for methods see: Lindholm et al. 2007, Voutilainen et al. 2011)

Purification: Purified by ion-exchange chromatography and gel-filtration from serum-free CHO growth media.
Protein is sterile-filtrated through 0.22 µg filter.
Purified human CDNF is characterized as homogenous material by mass-spectroscopy.

niveau d'endotoxine: Less than 1EU/µg of protein as determined by LAL method

Information d'application

Commentaires:

Synonyms: Cerebral dopamine neurotrophic factor - CDNF, ARMETL1.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/ml Concentration of the protein is determined by BCA Protein Analysis kit (Pierce). BSA was used as a standard

Buffer: PBS pH 7.5

Conseil sur la manipulation: Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Détail du antigène

Antigène: CDNF (Cerebral Dopamine Neurotrophic Factor (CDNF))

Autre désignation: CDNF (CDNF Produits)

Sujet: CDNF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- (Lindholm et al. 20007, Voutilainen et al., 2011) and MPTP-induced degeneration (Airavaara et al., 2012) of dopamine neurons in rodent models of Parkinson’s disease. When administered after 6-OHDA or MPTP –lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta.

Poids moléculaire: Approximately 18.5 kDa, a single non-glycosylated polypeptide chain containing 163 amino acids.

UniProt: Q49AH0