Protein L Protein (HRP)

N° du produit ABIN1536529

Détail du produit

Antigène: Protein L

Origine: Peptostreptococcus magnus

Hôte: Veuillez nous consulter SVP

Purification/Conjugué: Cette Protein L protéine est marqué à la HRP.

Application: Western Blotting (WB), Dot Blot (DB), Immunohistochemistry (IHC), ELISA

Information d'application

Indications d'application: The ideal working concentrations for each specific application should be determined by titration assay. Appropriate concentrations will be affected by several factors including primary antibody, antigen amount, sensitivity of detection method, temperature, and incubation time. The following dilution ranges are recommended starting points: Dot blot: 1:2,000-1:20,000 Western blot: 1:2,000-1:20,000 ELISA: 1:20,000-1:200,000 Other applications: user-optimized

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/ml

Buffer: PBS (pH 7.4) containing 10 mg/ml BSA and 0.02% sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: The reagent may be stored at 4°C for short periods of up to one-month. For longer periods, aliquot and store at the reagent at -20°C for longer storage. Avoid repeated freeze/thaw cycles. If slight precipitation occurs upon prolonged storage, clarify the solution by centrifugation before use.

Détail du antigène

Antigène: Protein L

Abstract: Protein L Produits

Sujet: Protein L is a cell surface protein expressed by Peptostreptoccocus magnus, that binds to the variable light chains (kappa chains) of immunoglobulins without interfering with antigen binding. In contrast to IgG-binding proteins such as protein A and protein G, protein L can be used for the detection and purification of mammalian kappa light chain antibodies of all classes. HRP-Protein L is produced by covalently linking HRP to protein L. The complex can be used in immuno-detection such as immunoblotting (western or dot blot), ELISA, and immunohistochemistry.