VEGF189 Protein (Homodimer)

N° du produit ABIN1589577

Détail du produit

Antigène: VEGF189 (Vascular Endothelial Growth Factor 189 (VEGF189))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: Homodimer

Origine: Humain

Source: Escherichia coli (E. coli)

Fonction: VEGF189

Séquence: APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQEKKSVRG KGKGQKRKRK KSRYKSWSVP CGPCSERRKH LFVQDPQTCK CSCKNTDSRC KARQLELNER TCRCDKPRR

Specificité: Chromosomal location:6p12

Attributs du produit: Length (aa):189

Pureté: > 98 % by SDS-PAGE

niveau d'endotoxine: < 0.1 ng per μg of human VEGF189

Information d'application

Indications d'application: Measured by the stimulation of cell proliferation in human umbilical vein endothelial cells in the range of 2-20 ng/mL.

Commentaires:

Cytokines & Growth Factors

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: The lyophilized VEGF189 is soluble in water and most aqueous buffers. The lyophilized VEGF189 should be reconstituted in PBS or medium containing at least 0.1 % human or bovine serum albumin to a concentration not lower than 50 μg/mL.

Buffer: 50 mM acetic acid

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: RT,-20 °C

Stockage commentaire: The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20°C. Reconstituted VEGF189 should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.

Détail du antigène

Antigène: VEGF189 (Vascular Endothelial Growth Factor 189 (VEGF189))

Abstract: VEGF189 Produits

Sujet: Human Vascular Endothelial Growth Factor VEGF189, a 21 kDa protein consisting of 189 amino acid residues, is produced as a homodimer. VEGF is a polypeptide growth factor and a member of the platelet-derived growth factor family. It is a specific mitogen for vascular endothelial cells and a strong angiogenic factor in vivo. Two high-affinity tyrosine kinase receptors for VEGF165 have been identified, VEGFR-1 (FLT-1), and VEGFR-2 (KDR). Consistent with the endothelial cell-specific action of VEGF165, expression of both receptor genes has been found predominantly but not exclusively on endothelial cells. Expression of VEGFR-1 was also found on human monocytes, neutrophils (PMNs), bovine brain pericytes and villous and extra villous trophoblast. In addition to its action as a mitogen it is a potent vasular permeability factor (VPF) in vivo. VEGF165 is also a chemo attractant molecule for monocytes and endothelial cells. 5 different proteins are generated by diffential splicing: VEGF121, VEGF145, VEGF165, VEGF189 and VEGF206. The most abundant form is VEGF165. Where¬as VEGF121 and VEGF165 are secreted proteins, VEGF145, VEGF189 and VEGF206 are strongly cell-associated. The isoforms VEGF145, VEGF165 and VEGF189 bind to heparin with high affinity. VEGF165 is appa¬rently a homo-dimer, but preparations of VEGF165 show some heterogeneity on SDS gels, depending on the secretion of different glycosylation patterns. All dimeric forms have similar biological activities but their bio¬availability is very different. There is good evidence that heterodimeric molecules between the different isoforms also exists and that different cells and tissues express different VEGF isoforms. The other members of this in¬creasing growth factor family are VEGF-B, -C, -D and -E. Another member is the Placenta growth factor PlGF.
Synonyms: vascular endothelial growth factor A, VEGFA,VPF, VEGF, MVCD1

Poids moléculaire: ~ 42.0 kDa

ID gène: 7422

NCBI Accession: NM_001025366, NP_001020537

UniProt: P15692