HGF Protein (Heterodimer)

N° du produit ABIN1589618

Détail du produit

Antigène: HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: Heterodimer

Origine: Humain

Source: Cellules d'insectes

Séquence: QRKRRNTIHE FKKSAKTTLI KIDPALKIKT KKVNTADQCA NRCTRNKGLP FTCKAFVFDK ARKQCLWFPF NSMSSGVKKE FGHEFDLYEN KDYIRNCIIG KGRSYKGTVS ITKSGIKCQP WSSMIPHEHS YRGKDLQENY CRNPRGEEGG PWCFTSNPEV RYEVCDIPQC SEVECMTCNG ESYRGLMDHT ESGKICQRWD HQTPHRHKFL PERYPDKGFD DNYCRNPDGQ PRPWCYTLDP HTRWEYCAIK TCADNTMNDT DVPLETTECI QGQGEGYRGT VNTIWNGIPC QRWDSQYPHE HDMTPENFKC KDLRENYCRN PDGSESPWCF TTDPNIRVGY CSQIPNCDMS HGQDCYRGNG KNYMGNLSQT RSGLTCSMWD KNMEDLHRHI FWEPDASKLN ENYCRNPDDD AHGPWCYTGN PLIPWDYCPI SRCEGDTTPT IVNLDHPVIS CAKTKQLRVV NGIPTRTNIG WMVSLRYRNK HICGGSLIKE SWVLTARQCF PSRDLKDYEA WLGIHDVHGR GDEKCKQVLN VSQLVYGPEG SDLVLMKLAR PAVLDDFVST IDLPNYGCTI PEKTSCSVYG WGYTGLINYD GLLRVAHLYI MGNEKCSQHH RGKVTLNESE ICAGAEKIGS GPCEGDYGGP LVCEQHKMRM VLGVIVPGRG CAIPNRPGIF VRVAYYAKWI HKIILTYKVP QS

Attributs du produit: Length (AA): 692
Chromosomal location: 7q21.1
The activity was assayed for scattering activity in the MDCK cell assay. The ED50 was for this effect is typically at 1.0-5.0 ng/mL.

Pureté: > 95 % by SDS-PAGE. Visualized by silver stain

Information d'application

Commentaires:

Cytokines & Growth Factors

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: The lyophilized human HGF is soluble in water and acetic acid (50 mM) and can be reconstituted to a concentration of 100 μg/mL. Further dilutions should be made into buffer containing protein or medium containing serum.

Buffer: 50 mM acetic acid

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: 0 °C

Stockage commentaire: The lyophilized HGF, though stable at room temperature, is best stored desiccated below 0 °C. Reconstituted it should be stored in working aliquots at -20 °C to -70 °C.

Référence

Rezzola, Dal Monte, Belleri, Bugatti, Chiodelli, Corsini, Cammalleri, Cancarini, Morbidelli, Oreste, Bagnoli, Semeraro, Presta: "Therapeutic Potential of Anti-Angiogenic Multitarget N,O-Sulfated E. Coli K5 Polysaccharide in Diabetic Retinopathy." dans: Diabetes, Vol. 64, Issue 7, pp. 2581-92, (2015) (PubMed).

Lohr, Mock, Beckhove, Herold-Mende: "Endothelial Cells Derived from Non-malignant Tissues Are of Limited Value as Models for Brain Tumor Vasculature." dans: Anticancer research, Vol. 35, Issue 5, pp. 2681-90, (2015) (PubMed).

Détail du antigène

Antigène: HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))

Autre désignation: HGF (HGF Produits)

Synonymes: DFNB39 Protein, F-TCF Protein, HGFB Protein, HPTA Protein, SF Protein, HGF Protein, C230052L06Rik Protein, HGF/SF Protein, NK1 Protein, NK2 Protein, SF/HGF Protein, hepatocyte growth factor Protein, HGF Protein, Hgf Protein

Sujet: Human Hepatocyte Growth Factor (HGF), also known as scatter factor, is a pleiotropic cytokine that shows homology to the enzymes of the blood coagulation cascade. It stimulates the motility and invasion of several cancer cell types and can induce angiogenesis. Recently HGF was found to be identical to scatter factor, a fibroblast-derived factor promoting the dissociation of epithelial and vascular endothelial cell colonies in monolayer cell cultures by stimulating cell migration. HGF is synthesized as a biologically inactive single chain precursor, which is cleaved by a specific, extracellular serum serine protease to a fully active heterodimer. This mature, biologically active HGF consists of a disulfide-linked alpha-beta heterodimer of the two cleavage products. Previous studies have shown that single chain and heterodimeric HGF are equally active in vitro assay systems due to either production of the serine protease in cell culture or the presence of the ubiquitous protease in serum. All biological responses induced by HGF are elicited by binding to its transmembrane tyrosine kinase receptor, which is encoded by the MET proto-oncogene. After autophosphorylation of the receptor different cytoplasmic effectors are activated that bind to the same multifunctional docking site of the receptor. HGF function is essential for normal development. Knockout studies have demonstrated that both ligand and receptor deficient mice display an embryonic lethal phenotype. Hepatocytes have to be primed before they can fully respond to HGF. This priming requires cytokines as TNF and IL-6. Recent studies have suggested that HGF synergizes with basic FGF in the induction of angiogenesis.
Synonyms: HGF, SF, HGFB, HPTA, F-TCF, DFNB39, Hepatocyte growth factor, Scatter factor

Poids moléculaire: 78.0 kDa

NCBI Accession: NM_000601, NP_000592

UniProt: P14210

Pathways: Signalisation RTK, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor