KIT Ligand Protein (KITLG)

N° du produit ABIN1589652

Détail du produit

Antigène: KIT Ligand (KITLG)

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Souris

Source: Escherichia coli (E. coli)

Fonction: SCF

Séquence: MKEICGNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV IQLSLSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVLC MEENAPKNIK ESPKRPETRS FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA

Specificité: Chromosomal location:10 D1, 10 57.0 cM

Attributs du produit: Length (aa):165

Pureté: > 95 % by SDS-PAGE

niveau d'endotoxine: < 0.1 ng per μg of mSCF

Information d'application

Indications d'application: The ED50 as determined by the dose-dependent stimulation of the proliferation of the human TF-1 cell line is in the range of 2-10 ng/mL.

Commentaires:

Cytokines & Growth Factors

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge vial prior to opening. Mouse SCF should be reconstituted in 50 mM acetic acid or water to a concentration of 0.1 mg/mL. This solution can be diluted in water or other buffer solutions or stored at -20 °C.

Buffer: 50 mM aceetic acid

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles. Freeze/thaw cycles will result in significant loss of activity.

Stock: 0 °C

Stockage commentaire: The lyophilized mouse SCF is best stored desiccated below 0°C. Freeze/thaw cycles will result in significant loss of activity. Avoid repeated freeze-thaw cycles.

Détail du antigène

Antigène: KIT Ligand (KITLG)

Autre désignation: SCF (KITLG Produits)

Sujet: Stem cell factor (SCF), also known as ckit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28-40 kDa type I transmembrane glycoprotein. It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors. SCF is a primary growth and activation factor for mast cells and eosinophils. Mature mouse SCF consists of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 36 aa cytoplasmic tail. Proteolytic cleavage at two alternate sites in the extracellular juxtamembrane region releases a 25 kDa soluble. An alternately spliced isoform of mouse SCF lacks 28 aa that encompasses the primary proteolytic recognition site. Within the ECD of the short isoform, mouse SCF shares 93% aa sequence identity with rat SCF and 72 %-75 % with canine, feline, and human SCF. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells. Noncovalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF R/ckit to trigger receptor dimerization and signaling.
Synonyms: Kitl, Gb, SF, Sl, Clo, Con, Mgf, SCF, SLF, Kitlg, contrasted

Poids moléculaire: 18.42 kDa

ID gène: 17311

NCBI Accession: NM_013598, NP_038626

UniProt: P20826

Pathways: Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway