VEGFC Protein (Cys152Ser-Mutant) (His tag)
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- Antigène Voir toutes VEGFC Protéines
- VEGFC (Vascular Endothelial Growth Factor C (VEGFC))
- Type de proteíne
- Recombinant
- Activité biologique
- Active
- Attributs du protein
- Cys152Ser-Mutant
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Origine
- Rat
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Source
- Cellules d'insectes
- Purification/Conjugué
- Cette VEGFC protéine est marqué à la His tag.
- Fonction
- VEGF-C152S
- Séquence
- DTVKLAAAHY NTEILKSIDN EWRKTQCMPR EVCIDVGKEF GAATNTFFKP PSVSVYRCGG CCNSEGLQCM NTSTGYLSKT LFEITVPLSQ GPKPVTISFA NHTSCRCMSK LDVYRQVHSI IHHHHHH
- Specificité
- Chromosomal location:16p11
- Attributs du produit
- Length (aa):127
- Pureté
- > 90 % by SDS-PAGE
- Top Product
- Discover our top product VEGFC Protéine
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- Indications d'application
- (A) The proliferative response to rrVEGF-CC152S was assayed in VEGFR3-expressing porcine aortic endothelial (PAE) cells (in vitro). (B) The lymphangiogenic response to rrVEGF-CC152S loaded in a biopolymeric albumin-alginate microcapsules for targeted slow-release was assayed in male Wistar rats.
- Commentaires
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Cytokines & Growth Factors
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- The lyophilized VEGF-C152S is soluble in water and most aqueous buffers. The lyophilized VEGF-C152S should be reconstituted in PBS or medium to a concentration not lower than 50 μg/mL.
- Buffer
- 50 mM acetic acid
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -20 °C,-80 °C
- Stockage commentaire
- Lyophilized samples are stable for greater than six months at -20°C to -70°C. Reconstituted VEGF-C152S should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles!
- Date de péremption
- 6 months
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- Antigène
- VEGFC (Vascular Endothelial Growth Factor C (VEGFC))
- Autre désignation
- VEGF-C152S (VEGFC Produits)
- Synonymes
- VEGFC Protein, vegf-c Protein, xvegf-c Protein, Flt4-L Protein, VRP Protein, AW228853 Protein, VEGF-C Protein, vascular endothelial growth factor c Protein, vascular endothelial growth factor C Protein, vascular endothelial growth factor C S homeolog Protein, vegfc Protein, VEGFC Protein, vegfc.S Protein, Vegfc Protein
- Sujet
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VEGF-C152S is a point mutant generated by the replacement of the second conserved Cys residue of the recombinant processed VEGF-C by a Ser residue. VEGF-C152S is analog to the human VEGF-C156S mutant and only active toward VEGFR-3/FLT-4 but, unlike wild type VEGF-C, is unable to bind to and to activate signalling through VEGFR-2/KDR. VEGF-C152S was inactive in the vascular permeability assay and did not increase migration of the capillary endothelial cells, indicating that these VEGF-like effects of VEGF-C require VEGFR-2 binding. VEGF-C, also known as Vascular Endothelial Growth Factor Related Protein (VRP), is a recently discovered VEGF growth factor family member that is most closely related to VEGF-D. The rat VEGF-C cDNA encodes a pre-pro-protein of 416 amino acids residues. It is almost identical to the mouse VEGF-C protein. Similar to VEGF-D, VEGF-C has a VEGF homology domain spanning the middle third of the precursor molecule and long N- and C-terminal extensions. In adults, VEGF-C is highly expressed in heart, placenta, ovary and small intestine. Recombinant rat VEGF-C, lacking the N- and C-terminal extensions and containing only the middle VEGF homology domain, forms primarily non-covalently linked dimers. This protein is a ligand for both VEGFR-2/KDR and VEGFR-3/FLT-4. Since VEGFR-3 is strongly expressed in lymphatic endothelial cells, it has been postulated that VEGF-C is involved in the regulation of the growth and/or differentiation of lymphatic endothelium. Although recombinant rat VEGF-C is also a mitogen for vascular endothelial cells, it is much less potent than VEGF-A. The recombinant rat VEGF-C contains 127 amino acids residues and was fused to a His-tag (6x His) at the C-terminal end. As a result of glycosylation VEGF-C migrates as an 18-24 kDa protein in SDS-PAGE under reducing conditions.
Synonyms: vascular endothelial growth factor C, Vegfc - Poids moléculaire
- 18.0 - 24.0 kDa
- ID gène
- 114111
- NCBI Accession
- NM_053653, NP_446105
- UniProt
- O35757
- Pathways
- Signalisation RTK, Signaling Events mediated by VEGFR1 and VEGFR2
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