CD34 Protein (CD34) (C-Term, Extracellular Domain)

N° du produit ABIN1684672

Détail du produit

Antigène: CD34

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: C-Term, Extracellular Domain

Origine: Souris

Source: HEK-293T Cells

Specificité: Optimized DNA sequence encoding extracellular domain of mouse CD34 including a C-terminal 6His tag was expressed in HEK293 cells.

Attributs du produit: Recombinant mouse CD34 is a monomer protein consisting of70 amino acid residue subunits, due to glycosylation migrates as an approximately 50 kDa protein on SDS-PAGE.

Pureté: > 95 %, as determined by SDS-PAGE and HPLC

Stérilité: 0.2 μm filtered

niveau d'endotoxine: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

Information d'application

Commentaires:

Biologically active: Measured by the ability to support the adhesion of the HUVEC cell line. Coated plates with immobilized recombinant mouse CD34 ( 0.8 µg/mL) are able to adhere 40 % of cells ( 4 x 104 cells/well) after one hour at 37 °C

Restrictions: For Research Use only

Stockage

Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers

Buffer: PBS solution, pH7.2.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.

Stock: -20 °C

Stockage commentaire: The lyophilized protein is stable for at least years from date of receipt at -20 °C. Upon reconstitution, this cytokine can be stored in working aliquots at - 8 °C for one month, or at -20 °C for six months, with a carrier protein without detectable loss of activity.

Date de péremption: 12-24 months

Détail du antigène

Antigène: CD34

Autre désignation: CD34 (CD34 Produits)

Synonymes: AU040960 Protein, CD34 molecule Protein, CD34 antigen Protein, CD34 Protein, Cd34 Protein

Sujet: Parathyroid hormone is a circulating hormone that acts as the central regulator of calcium metabolism by directly targeting bone, kidney, and intestine. The classical concept of PTH action is that it regulates serum calcium levels by stimulating bone resorption, however, intermittent administration of PTH selectively stimulates bone formation. It is now known that PTH binds to its receptor PTH1R, and activates the G protein alpha subunits. Besides PKA and PKC activation, PTH also regulates MAPKs including p42/p44 ERKs, p38, and c-Jun N-terminal kinase subtypes.

UniProt: Q64314