HSP70 Protein (full length)

N° du produit ABIN1686685

Détail du produit

Antigène: HSP70 (Heat Shock Protein 70 (HSP70))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: full length

Origine: Humain

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), Western Blotting (WB), Functional Studies (Func), Activity Assay (AcA), ELISA

Séquence: MAKAAAIGID LGTTYSCVGV FQHGKVEIIA NDQGNRTTPS YVAFTDTERL IGDAAKNQVA LNPQNTVFDA KRLIGRKFGD PVVQSDMKHW PFQVINDGDK PKVQVSYKGE TKAFYPEEIS SMVLTKMKEI AEAYLGYPVT NAVITVPAYF NDSQRQATKD AGVIAGLNVL RIINEPTAAA IAYGLDRTGK GERNVLIFDL GGGTFDVSIL TIDDGIFEVK ATAGDTHLGG EDFDNRLVNH FVEEFKRKHK KDISQNKRAV RRLRTACERA KRTLSSSTQA SLEIDSLFEG IDFYTSITRA RFEELCSDLF RSTLEPVEKA LRDAKLDKAQ IHDLVLVGGS TRIPKVQKLL QDFFNGRDLN KSINPDEAVA YGAAVQAAIL MGDKSENVQD LLLLDVAPLS LGLETAGGVM TALIKRNSTI PTKQTQIFTT YSDNQPGVLI QVYEGERAMT KDNNLLGRFE LSGIPPAPRG VPQIEVTFDI DANGILNVTA TDKSTGKANK ITITNDKGRL SKEEIERMVQ EAEKYKAEDE VQRERVSAKN ALESYAFNMK SAVEDEGLKG KISEADKKKV LDKCQEVISW LDANTLAEKD EFEHKRKELE QVCNPIISGL YQGAGGPGPG GFGAQGPKGG SGSGPTIEEV D

Specificité: ~70 kDa

Attributs du produit: The protein has ATPase activity at the time of manufacture of 3.0 µM phosphate liberated/hr/μg protein in a 200 µL reaction at 37 °C (pH 7.5) in the presence of 20 µL of 1 mM ATP using a Malachite Green assay.

Purification: Multi-Step Purified

Pureté: >95%

Biological Activity Comment: ATPase active

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

This product has been certified >95% pure using SDS-PAGE analysis. The protein has ATPase activity at the time of manufacture of 3.0μM phosphate liberated/hr/μg protein in a 200μl reaction at 37°C (pH7.5) in the presence of 20ul of 1mM ATP using a Malachite Green assay.

Restrictions: For Research Use only

Stockage

Concentration: Lot specific

Buffer: 50 mM Tris/HCl pH 7.5, 2.5 mM Bme, 0.15M NaCl, 10 % glycerol

Stock: -20 °C

Détail du antigène

Antigène: HSP70 (Heat Shock Protein 70 (HSP70))

Autre désignation: Hsp70 (HSP70 Produits)

Synonymes: APG-2 Protein, HS24/P52 Protein, HSPH2 Protein, RY Protein, hsp70 Protein, hsp70RY Protein, CG31354 Protein, HSP70 Protein, Hsp70Bb Protein, hsp70B Protein, hsp70Bb-prime Protein, DmelCG5834 Protein, CG5834 Protein, HSPA1 Protein, HSP70B' Protein, HSPA6 Protein, ARABIDOPSIS HEAT SHOCK PROTEIN 70 Protein, ATHSP70 Protein, heat shock protein 70 Protein, LOC100305036 Protein, hsc70 Protein, Hsp70 Protein, Hsp70-1 Protein, Hsp70.1 Protein, hsp68 Protein, Hsp110 Protein, irp94 Protein, HSP70-2 Protein, HSPA1B Protein, HSPA2 Protein, hsp70-5 Protein, HSP70-1 Protein, HSP70.1 Protein, HSP70.2 Protein, heat shock protein family A (Hsp70) member 4 Protein, CG5834 gene product from transcript CG5834-RA Protein, heat shock protein 70 Protein, heat shock protein family A (Hsp70) member 6 Protein, heat shock 70kDa protein 2 Protein, heat shock 70 kD protein cognate Protein, Hsp70 family chaperone Protein, Heat shock protein 70 Protein, Heat shock protein 70, putative Protein, heat shock protein 1B Protein, heat shock protein family A member 4 Protein, heat shock 70kDa protein 1A Protein, heat shock protein 1 Protein, Heat Shock Protein Protein, heat shock cognate 70-kd protein Protein, Heat shock 70 kDa protein 1A Protein, HSPA4 Protein, Hsp70Bbb Protein, HSP70 Protein, HSPA6 Protein, HSPA2 Protein, PCC7424_2419 Protein, Isop_1041 Protein, CGB_C3390W Protein, Bacsa_1698 Protein, dnaK-B Protein, LOC100305036 Protein, Hspa1b Protein, Hspa4 Protein, HSPA1A Protein, hsp1 Protein, hsp-70 Protein, hsp70 Protein, LOC108348108 Protein

Sujet: HSP70 genes encode abundant heat-inducible 70- kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50 % identity (2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All HSP70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the HSP70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of HSP70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport. Looking for more information on HSP70? Visit our new HSP70 Scientific Resource Guide at http://www.HSP70.com.

Poids moléculaire: approx. 70 kDa

ID gène: 3303

NCBI Accession: NM_005345