PARP1 Enzyme is highly purified and enzymatically active human PARP1, expressed in a baculovirus expression system. It is useful for high-throughput enzymatic assays, visualization of the automodification reaction by SDS-PAGE and Western blotting, ELISA, standard for SDS-PAGE and WB, and in other assays. Full-length hPARP1
PARP1
Origine: Humain
Hôte: Escherichia coli (E. coli)
Recombinant
> 95 %
SDS, WB, Imm, PC
Indications d'application
10 ng/reaction
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Reconsitute in dH2O
Stock
-20 °C
Cinque, Ferino, Pedersen, Xodo: "Role of Poly [ADP-ribose] Polymerase 1 in Activating the Kirstenras (KRAS) Gene in Response to Oxidative Stress." dans: International journal of molecular sciences, Vol. 21, Issue 17, (2020) (PubMed).
Zhang, Wang, Ding, Yu: "Site-specific characterization of the Asp- and Glu-ADP-ribosylated proteome." dans: Nature methods, Vol. 10, Issue 10, pp. 981-4, (2013) (PubMed).
Poly (ADP-ribose) polymerase-1 (PARP1) is an abundant and ubiquitous nuclear enzyme that catalyzes the NAD(+)-dependent addition of ADP-ribose polymers on a variety of nuclear proteins.