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Leptin Protein (LEP) (AA 22-167)

LEP Origine: Rat Hôte: Escherichia coli (E. coli) Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
N° du produit ABIN2018192
  • Antigène Voir toutes Leptin (LEP) Protéines
    Leptin (LEP)
    Type de proteíne
    Recombinant
    Activité biologique
    Active
    Attributs du protein
    AA 22-167
    Origine
    • 12
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Rat
    Source
    • 30
    • 20
    • 2
    • 1
    • 1
    Escherichia coli (E. coli)
    Attributs du produit
    ED50 < 10 μg/mL, measured by a cell proliferation assay using LoVo cells, corresponding to a specific activity of > 100 units/mg.
    AA 22-167, expressed with an N-terminal Met.
    Pureté
    > 95 % as analyzed by SDS-PAGE and HPLC.
    niveau d'endotoxine
    < 0.2 EU/μg, determined by LAL method.
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    Discover our top product LEP Protéine
  • Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Reconstituted in ddH2O at 100 μg/mL.
    Buffer
    Lyophilized after extensive dialysis against 50 mM Tris, pH 8.0.
    Stock
    -80 °C
    Stockage commentaire
    Lyophilized recombinant rat Leptin (rrLeptin) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rrLeptin remains stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.
    Date de péremption
    6 months
  • Antigène
    Leptin (LEP)
    Autre désignation
    Leptin (LEP Produits)
    Synonymes
    ob Protein, obese Protein, LEPD Protein, OB Protein, OBS Protein, leptin Protein, Lep Protein, LEP Protein, lep Protein
    Sujet
    Leptin is a cytokine belonging to the Interleukin 6 family, and has a four-helix bundle structure. Leptin is encoded by the ob gene, and produced and secreted by white adipose tissue. The receptors of Leptin are Type I cytokine receptors, which exist in two different forms: a short form expressed in multiple tissues, and a long form expressed exclusively in the central nervous system (CNS). Upon binding to Leptin, the receptors activate the JAK/STAT3 pathway and PI3K, and stimulate transcriptional programs that regulate feeding behavior, metabolic rate, endocrine axes, and glucose fluxes. The deficiency of Leptin in human and mouse causes morbid obesity, diabetes, and neuroendocrine anomalies. Leptin also has effects on reproduction and immunity. In summary, Leptin is a pivotal cytokine controlling energy balance, and as such has profound effects on human health. Recombinant rat Leptin (rrLeptin) produced in E. coli is a single non-glycosylated polypeptide chain containing 147 amino acids. A fully biologically active molecule, rrLeptin has a molecular mass of 16.3 kDa analyzed by reducing SDS-PAGE.
    Synonyms: Obesity protein (OB)
    Poids moléculaire
    16.3 kDa, observed by reducing SDS-PAGE.
    UniProt
    P50596
    Pathways
    Signalistation JAK/STAT, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process
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