KITLG
Origine: Humain
Hôte: Escherichia coli (E. coli)
Recombinant
Greater than 95.0 % as determined by the following methods:(a) Analysis by SEC-HPLC(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel
Active
The ED50, as determined by the dose-dependant stimulation of human TF-1 cells, is < 2 ng/mL, corresponding to a specific activity of 5x10^5 IU/mg. The sequence of the first five N-terminal amino acids has been found to be Met-Glu-Gly-Ile-Cys.
Pureté
Greater than 95.0 % as determined by the following methods:(a) Analysis by SEC-HPLC(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel
KITLG
Origine: Humain
Hôte: HEK-293 Cells
Recombinant
The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
It is recommended that the lyophilized recombinant human stem cell factor (SCF) be reconstituted in sterile 18 M omega-cm H2O not less than 100 μg/mL, which can then be further diluted to other aqueous solutions.
Buffer
The protein was lyophilized after extensive dialysis against 20 mM HAc-NaAc, pH 4.5, 200 mM NaCl buffer.
Conseil sur la manipulation
Avoid repeated freeze-thaw cycles.
Stock
-20 °C
Stockage commentaire
Lyophilized recombinant human stem cell factor (SCF) remains stable at room temperature for three weeks, but it is best stored desiccated below -18 °C. Upon reconstitution Recombinant Human Stem Cell Factor (SCF) should be stored at 4 °C for up to seven days. For long term storage it is recommended that a carrier protein (0.1% HSA or BSA ) be added.
Herraiz, Journé, Abdel-Malek, Ghanem, Jiménez-Cervantes, García-Borrón: "Signaling from the human melanocortin 1 receptor to ERK1 and ERK2 mitogen-activated protein kinases involves transactivation of cKIT." dans: Molecular endocrinology (Baltimore, Md.), Vol. 25, Issue 1, pp. 138-56, (2011) (PubMed).
Woost, Solchaga, Meyerson, Shankey, Goolsby, Jacobberger: "High-resolution kinetics of cytokine signaling in human CD34/CD117-positive cells in unfractionated bone marrow." dans: Blood, Vol. 117, Issue 15, pp. e131-41, (2011) (PubMed).
Human Stem Cell Factor (SCF), produced in E. coli is a single, non-glycosylated polypeptide chain containing 165 amino acids and having a molecular mass of 18,409 Da. Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity during the early stages of hematopoiesis. Stem Cell Factor (SCF) stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors. Synonyms: rHuSCF, SCF