Each Investigator should determine their own optimal working dilution for specific applications.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Resonstitute 1:1 w/v distilled water.
Buffer
Reconstituted Solution will contain 1 mg/mL RAP protein in Tris buffer saline, pH 7.5, 0.1 % BSA, 0.09 % Sodium Azide.
Précaution d'utilisation
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Conseil sur la manipulation
Avoid repeated freeze/thaw cycles. Ligand binding to RAP is calcium-dependent. Lipidreceptors may be released from RAP by using a buffer containing 10 mM EDTA. Buffers containing phosphate should not be used as it forms precipitates with calcium.
Stock
4 °C/-20 °C
Stockage commentaire
Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.
Antigène
LRPAP1
(Low Density Lipoprotein Receptor-Related Protein Associated Protein 1 (LRPAP1))
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Sujet
Low density lipoprotein receptor-related protein associated protein 1 also known as LRPAP1 or RAP is a chaperone protein which in humans is encoded by the LRPAP1 gene. RAP is involved with trafficking of certain members of the LDL receptor family including LRP1 and LRP2. Alternative Names: Receptor-associated Protein recombinant protein, Rat RAP protein