Cette Cathepsin B protéine est marqué à la His tag.
Séquence
AA 18-339
Attributs du produit
rh CTSB is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 36.7 kDa (pro-form) and 29 kDa (mature-form). The predicted N-terminus is Arg18 (pro-form) or Phe74 (mature-form). Due to glycosylation, The reducing (R) protein migrates as 43 kDa (pro-form) band in SDS-PAGE and there may be a 34 kDa (mature-form) band.
CTSB
Origine: Souris
Hôte: Human Cells
Recombinant
> 95 % as determined by reducing SDS-PAGE.
Restrictions
For Research Use only
Format
Lyophilized
Buffer
50 mM Tris, 150 mM NaCl, pH 8.0
Conseil sur la manipulation
Please avoid repeated freeze-thaw cycles.
Stock
-20 °C
Stockage commentaire
No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Hartmann, Lopez Cruz, Alameh, Ho, Rabideau, Pentelute, Bradley, Martchenko: "Characterization of Novel Piperidine-Based Inhibitor of Cathepsin B-Dependent Bacterial Toxins and Viruses." dans: ACS infectious diseases, Vol. 4, Issue 8, pp. 1235-1245, (2018) (PubMed).
Zilbermintz, Leonardi, Jeong, Sjodt, McComb, Ho, Retterer, Gharaibeh, Zamani, Soloveva, Bavari, Levitin, West, Bradley, Clubb, Cohen, Gupta, Martchenko: "Identification of agents effective against multiple toxins and viruses by host-oriented cell targeting." dans: Scientific reports, Vol. 5, pp. 13476, (2015) (PubMed).
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Sujet
Cathepsin B (CTSB) is also known as APP secretase (APPS) and CPSB, is an enzymatic protein belonging to the peptidase C1 family. Cathepsin B / CTSB is synthesized as a preproenzyme. Following removal of the signal peptide, the inactive proenzyme undergoes further modifications including removal of the pro region to result in the active enzyme. The catalytic activity of Cathepsin B / APPS contains: Hydrolysis of proteins with broad specificity for peptide bonds, Preferentially cleaves -Arg-Arg-|-Xaa bonds in small molecule substrates (thus differing from cathepsin L), In addition to being an endopeptidase, shows peptidyl-dipeptidase activity, liberating C-terminal dipeptides. As a thiol protease, cathepsin B / CPSB is believed to participate in intracellular degradation and turnover of proteins and has also been implicated in tumor invasion and metastasis. Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors.