This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 71.5 kDa. The protein migrates as 100-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
ERBB3
Origine: Humain
Hôte: HEK-293 Cells
Recombinant
The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
ERBB3
Origine: Humain
Hôte: Mammalian Cells
Recombinant
The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Restrictions
For Research Use only
Format
Lyophilized
Buffer
PBS, pH 7.4
Conseil sur la manipulation
Please avoid repeated freeze-thaw cycles.
Stock
-20 °C
Stockage commentaire
No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
DAgostino, Gentile, Ponziani, Di Vittorio, Dituri, Giannelli, Rossi, Marzullo, Giansanti, De Laurenzi, Iacobelli, Ippoliti, Capone, Sala: "EV20‑sss‑vc/MMAF, an HER‑3 targeting antibody‑drug conjugate displays antitumor activity in liver cancer." dans: Oncology reports, Vol. 45, Issue 2, pp. 776-785, (2021) (PubMed).
Shinozaki, Hashimoto, Fukui, Uchiyama: "Efficient generation of single domain antibodies with high affinities and enhanced thermal stabilities." dans: Scientific reports, Vol. 7, Issue 1, pp. 5794, (2019) (PubMed).
Capone, Lamolinara, DAgostino, Rossi, De Laurenzi, Iezzi, Iacobelli, Sala: "EV20-mediated delivery of cytotoxic auristatin MMAF exhibits potent therapeutic efficacy in cutaneous melanoma." dans: Journal of controlled release : official journal of the Controlled Release Society, Vol. 277, pp. 48-56, (2019) (PubMed).
Sala, Rapposelli, Ghasemi, Piccolo, Traini, Capone, Rossi, Pelliccia, Di Risio, DEgidio, Tinari, Muraro, Iacobelli: "EV20, a Novel Anti-ErbB-3 Humanized Antibody, Promotes ErbB-3 Down-Regulation and Inhibits Tumor Growth In Vivo." dans: Translational oncology, Vol. 6, Issue 6, pp. 676-84, (2014) (PubMed).
ErbB3,also known as Her3 (human epidermal growth factor receptor 3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain. It therefore can bind the ligand but cannot mediate the intracellular signal transduction through protein phosphorylation. However, it does form heterodimers with ErbB2 or other EGFR members responsible for tyrosine phosphorylation to give a receptor complex and initiate the related pathway, which lead to cell proliferation or differentiation. Overexpression of this protein has been reported in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.