This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 41.7 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
FZD2
Origine: Humain
Hôte: HEK-293 Cells
Recombinant
>95 % as determined by SDS-PAGE.
Restrictions
For Research Use only
Format
Lyophilized
Buffer
Tris with Glycine, Arginine and NaCl, pH 7.5
Conseil sur la manipulation
Please avoid repeated freeze-thaw cycles.
Stock
-20 °C
Stockage commentaire
No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Henkel, Tatge, Schöttelndreier, Tao, Dong, Gerhard: "Receptor Binding Domains of TcdB from Clostridioides difficile for Chondroitin Sulfate Proteoglycan-4 and Frizzled Proteins Are Functionally Independent and Additive." dans: Toxins, Vol. 12, Issue 12, (2020) (PubMed).
Tao, Zhang, Meraner, Tovaglieri, Wu, Gerhard, Zhang, Stallcup, Miao, He, Hurdle, Breault, Brass, Dong: "Frizzled proteins are colonic epithelial receptors for C. difficile toxin B." dans: Nature, Vol. 538, Issue 7625, pp. 350-355, (2017) (PubMed).
Frizzled-2 (FZD2) is also known as FzE2, which belongs to the G-protein coupled receptor Fz/Smo family. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. FZD2 contains one FZ (frizzled) domain. FZD2 may be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. The Lys-Thr-X-X-X-Trp motif of FZD2 interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.