This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 97.7 kDa. The protein migrates as 130-140 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
ErbB2/Her2
Origine: Humain
Hôte: HEK-293 Cells
Recombinant
>90 % as determined by SDS-PAGE.
Active
Restrictions
For Research Use only
Format
Lyophilized
Buffer
PBS, pH 7.4
Conseil sur la manipulation
Please avoid repeated freeze-thaw cycles.
Stock
-20 °C
Stockage commentaire
No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Ding, Gangalum, Galstyan, Fox, Patil, Hubbard, Murali, Ljubimova, Holler: "HER2-positive breast cancer targeting and treatment by a peptide-conjugated mini nanodrug." dans: Nanomedicine : nanotechnology, biology, and medicine, Vol. 13, Issue 2, pp. 631-639, (2017) (PubMed).
Piechocki, Wu, Jones, Jacob, Gibson, Ethier, Abrams, Yagita, Venuprasad, Wei: "Induction of proapoptotic antibodies to triple-negative breast cancer by vaccination with TRAIL death receptor DR5 DNA." dans: International journal of cancer. Journal international du cancer, Vol. 131, Issue 11, pp. 2562-72, (2012) (PubMed).
Human Epidermal growth factor Receptor 2 (HER2) is also called ERBB2, HER-2,HER-2 /neu, NEU, NGL,TKR1 and c-erb B2,and is a protein giving higher aggressiveness in breast cancers. It is a member of the ErbB protein family, more commonly known as the epidermal growth factor receptor family. HER2 is a cell membrane surface-bound receptor tyrosine kinase and is normally involved in the signal transduction pathways leading to cell growth and differentiation. HER2 is thought to be an orphan receptor, with none of the EGF family of ligands able to activate it. Approximately 30 % of breast cancers have an amplification of the HER2 gene or overexpression of its protein product. Overexpression of this receptor in breast cancer is associated with increased disease recurrence and worse prognosis. HER2 appears to play roles in development, cancer, communication at the neuromuscular junction and regulation of cell growth and differentiation .