This protein carries no "tag". The protein has a calculated MW of 16.8 kDa. The protein migrates as 17 kDa,20 kDa and 24 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.
IFNG
Origine: Hamster
Hôte: Escherichia coli (E. coli)
Recombinant
Greater than 95 % by SDS-PAGE gel analyses
WB, ELISA
Restrictions
For Research Use only
Format
Lyophilized
Buffer
PBS, pH 7.4
Conseil sur la manipulation
Please avoid repeated freeze-thaw cycles.
Stock
-20 °C
Stockage commentaire
No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Ross, Pompano: "Diffusion of cytokines in live lymph node tissue using microfluidic integrated optical imaging." dans: Analytica chimica acta, Vol. 1000, pp. 205-213, (2018) (PubMed).
Razaghi, Villacrés, Jung, Mashkour, Butler, Owens, Heimann: "Improved therapeutic efficacy of mammalian expressed-recombinant interferon gamma against ovarian cancer cells." dans: Experimental cell research, Vol. 359, Issue 1, pp. 20-29, (2017) (PubMed).
Interferon-gamma (IFN-γ/IFNG) is a dimerized soluble cytokine that is the only member of the type II class of interferon. This interferon was originally called macrophage-activating factor, a term now used to describe a larger family of proteins to which IFN-γ belongs. IFN-gamma has been used in a wide variety of clinical indications. Interferon-gamma (IFNgamma) is a central regulator of the immune response and signals via the Janus Activated Kinase (JAK)-Signal Transducer and Activator of Transcription (STAT) pathway. Interferon gamma has broader roles in activation of innate and adaptive immune responses to viruses and tumors, in part through upregulating transcription of genes involved in cell cycle regulation, apoptosis, and antigen processing/presentation. Despite this, rodent and human trophoblast cells show dampened responses to IFNG that reflect the resistance of these cells to IFNG-mediated activation of major histocompatibility complex (MHC) class II transplantation antigen expression.