PCSK9
(Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9))
Type de proteíne
Recombinant
Activité biologique
Active
Attributs du protein
AA 31-692
Origine
Humain
Source
HEK-293 Cells
Purification/Conjugué
Cette PCSK9 protéine est marqué à la His tag.
Séquence
AA 31-692
Attributs du produit
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 75.1 kDa. The protein migrates as 20 kDa and 62 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation and proteolytic digestion.
PCSK9
Origine: Humain
Hôte: HEK-293 Cells
Recombinant
The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
PCSK9
Origine: Rat
Hôte: HEK-293 Cells
Recombinant
>95 % as determined by reduced SDS-PAGE.
Active
Restrictions
For Research Use only
Format
Lyophilized
Buffer
PBS, pH 7.4
Conseil sur la manipulation
Please avoid repeated freeze-thaw cycles.
Stock
-20 °C
Stockage commentaire
No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Lichtor, Chen, Elowe, Chen, Liu: "Side chain determinants of biopolymer function during selection and replication." dans: Nature chemical biology, Vol. 15, Issue 4, pp. 419-426, (2019) (PubMed).
Wijers, Zanoni, Liv, Vos, Jäckstein, Smit, Wilbrink, Wolters, van der Veen, Huijkman, Dekker, Kloosterhuis, van Dijk, Billadeau, Kuipers, Klumperman, von Eckardstein, Kuivenhoven, van de Sluis: "The hepatic WASH complex is required for efficient plasma LDL and HDL cholesterol clearance." dans: JCI insight, Vol. 4, Issue 11, (2019) (PubMed).
Grin, Dwivedi, Chathely, Trigatti, Prat, Seidah, Liaw, Fox-Robichaud: "Low-density lipoprotein (LDL)-dependent uptake of Gram-positive lipoteichoic acid and Gram-negative lipopolysaccharide occurs through LDL receptor." dans: Scientific reports, Vol. 8, Issue 1, pp. 10496, (2018) (PubMed).
Dugardin, Briand, Touche, Schonewille, Moreau, Le May, Groen, Staels, Lestavel: "Retrograde cholesterol transport in the human Caco-2/TC7 cell line: a model to study trans-intestinal cholesterol excretion in atherogenic and diabetic dyslipidemia." dans: Acta diabetologica, Vol. 54, Issue 2, pp. 191-199, (2017) (PubMed).
Susan-Resiga, Girard, Kiss, Essalmani, Hamelin, Asselin, Awan, Butkinaree, Fleury, Soldera, Dory, Baass, Seidah: "The Proprotein Convertase Subtilisin/Kexin Type 9-resistant R410S Low Density Lipoprotein Receptor Mutation: A NOVEL MECHANISM CAUSING FAMILIAL HYPERCHOLESTEROLEMIA." dans: The Journal of biological chemistry, Vol. 292, Issue 5, pp. 1573-1590, (2017) (PubMed).
Topchiy, Cirstea, Kong, Boyd, Wang, Russell, Walley: "Lipopolysaccharide Is Cleared from the Circulation by Hepatocytes via the Low Density Lipoprotein Receptor." dans: PLoS ONE, Vol. 11, Issue 5, pp. e0155030, (2017) (PubMed).
Boyd, Fjell, Russell, Sirounis, Cirstea, Walley: "Increased Plasma PCSK9 Levels Are Associated with Reduced Endotoxin Clearance and the Development of Acute Organ Failures during Sepsis." dans: Journal of innate immunity, Vol. 8, Issue 2, pp. 211-20, (2016) (PubMed).
Walley, Thain, Russell, Reilly, Meyer, Ferguson, Christie, Nakada, Fjell, Thair, Cirstea, Boyd: "PCSK9 is a critical regulator of the innate immune response and septic shock outcome." dans: Science translational medicine, Vol. 6, Issue 258, pp. 258ra143, (2015) (PubMed).
Antigène
PCSK9
(Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9))
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Sujet
Proprotein convertase subtilisin/kexin type 9 (PCSK9), is an enzyme which in humans is encoded by the PCSK9 gene. This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).