RAD51 Protéine

N° du produit ABIN2452204

Détail du produit

Antigène: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Functional Studies (Func)

Attributs du produit: This product was confirmed to possess single strand DNA stimulated ATPase activity.

Pureté: > 95 % as judged from SDS-PAGE analysis

Information d'application

Indications d'application: 1) Studies on homologous recombination in mammals including human
2) Studies on the interaction of Rad51 protein with various proteins
3) To be used as a standard for Western blotting

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 20 mM Tris-HCl pH 8.0, 100 mM KCl, 1 mM DTT, 0.5 mM EDTA, 10 % glycerol

Agent conservateur: Dithiothreitol (DTT)

Précaution d'utilisation: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C/-80 °C

Stockage commentaire: Store at -80 C for long period.

Référence

Murayama, Kurokawa, Mayanagi, Iwasaki: "Formation and branch migration of Holliday junctions mediated by eukaryotic recombinases." dans: Nature, Vol. 451, Issue 7181, pp. 1018-21, (2008) (PubMed).

Kurumizaka, Aihara, Kagawa, Shibata, Yokoyama: "Human Rad51 amino acid residues required for Rad52 binding." dans: Journal of molecular biology, Vol. 291, Issue 3, pp. 537-48, (1999) (PubMed).

Détail du antigène

Antigène: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Autre désignation: Rad51 (RAD51 Produits)

Synonymes: BRCC5 Protein, HRAD51 Protein, HsRad51 Protein, HsT16930 Protein, MRMV2 Protein, RAD51A Protein, RECA Protein, CRAD51 Protein, wu:fb38e12 Protein, zgc:77754 Protein, RGD1563603 Protein, ATRAD51 Protein, F22D1.20 Protein, F22D1_20 Protein, RAS associated with diabetes protein 51 Protein, AV304093 Protein, Rad51a Protein, Reca Protein, brcc5 Protein, rad51 Protein, rad51a Protein, reca Protein, xrad51 Protein, RAD51 recombinase Protein, RAS associated with diabetes protein 51 Protein, RAD51 recombinase L homeolog Protein, RAD51 Protein, rad51 Protein, Rad51 Protein, rad51.L Protein

Sujet: Human Rad51 protein is a functional and structural homolog of E. coli RecA protein, which plays a major role in genetic recombination and recombination repair by mediating strand exchange reaction between homologous DNA strands. Rad51 functionally and physically interacts with its paralogs Dmc1, Rad51B, Rad51C, Rad51D, Xrcc2 and Xrcc3, and also with Rad52 in recombination processes. It also interacts with oncogene proteins and tumor suppressors such as BRCA1, BRCA2, and P53 for the maintenance of genome stability. Rad51 protein was highly purified from E. coli over-expressing human Rad51 protein as a recombinant protein. Since the tag was removed from the recombinant protein (it still contains Gly-Ser-His at the N-terminal), it has been shown to retain nuclear filament forming and strand-exchange activity as well as interaction with Rad52.

UniProt: Q06609

Pathways: Réparation de l'ADN