Streptolysin O Protein (SLO)
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- Antigène Voir toutes Streptolysin O (SLO) Protéines
- Streptolysin O (SLO)
- Type de proteíne
- Recombinant
- Activité biologique
- Active
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Origine
- Streptococcus pyogenes
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Source
- Escherichia coli (E. coli)
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Attributs du produit
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Measurement of the activity: Definition of 1HU is activation of 50 % hemolysis using 3 % sheep red blood cells.
The product was highly purified from E.coli over-expressing SLO of Group C hemolytic streptococci. The specific activity is as high as 1,900,000 hemolytic units (HU) /mg and the product forms a big hole on the cell membrane, which enables the introduction of protein inside the cells in vivo. - Pureté
- > 98 % by SDS-PAGE
- Stérilité
- Sterile filtered
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- Indications d'application
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1) Antigen for the measurement of anti-streptolysin O antibody (ASO) (diagnostic reagent
2) Reagent for membrane pore formation to introduce small-to-macromolecules into living cells - Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
- Inactivated SLO can be reactivated by thiol reagents such as 20 mM cysteine or 10 mM DTT (2)
- Concentration
- 1 mg/mL
- Buffer
- PBS, 1 mM DTT, 50 % glycerol
- Agent conservateur
- Dithiothreitol (DTT)
- Précaution d'utilisation
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- -20 °C/-80 °C
- Stockage commentaire
- -20 C (For long term storage: -70 C)
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GPHR is a novel anion channel critical for acidification and functions of the Golgi apparatus." dans: Nature cell biology, Vol. 10, Issue 10, pp. 1135-45, (2008) (PubMed).
: "Delivery of proteins into living cells by reversible membrane permeabilization with streptolysin-O." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 6, pp. 3185-90, (2001) (PubMed).
: "The family of thiol-activated, cholesterol-binding cytolysins." dans: Toxicon : official journal of the International Society on Toxinology, Vol. 39, Issue 11, pp. 1681-9, (2001) (PubMed).
: "
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GPHR is a novel anion channel critical for acidification and functions of the Golgi apparatus." dans: Nature cell biology, Vol. 10, Issue 10, pp. 1135-45, (2008) (PubMed).
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- Antigène
- Streptolysin O (SLO)
- Autre désignation
- Streptolysin O (SLO Produits)
- Synonymes
- streptolysin O Protein, Streptolysin O Protein, Thiol-activated cytolysin Protein, slo Protein, M6_Spy0188 Protein
- Sujet
- Streptolysin O (SLO) is a membrane-damaging extracellular toxin produced by hemolytic streptococci. The membrane-damaging activity is measured by hemolysis of red-blood cells. SLO is easily inactivated in the presence of oxygen but can be reactivated by thiol compounds, so it is also called thiol-activated cytolysin. SLO is produced not only by Group A hemolytic streptococci but also by Group C and Group G strains. The amino acid sequences are highly conserved among them and their homology is over 98 %.
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