Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

Streptolysin O Protein (SLO)

SLO Origine: Streptococcus pyogenes Hôte: Escherichia coli (E. coli) Recombinant > 98 % by SDS-PAGE ELISA, WB, SDS Active
N° du produit ABIN2452205
  • Antigène Voir toutes Streptolysin O (SLO) Protéines
    Streptolysin O (SLO)
    Type de proteíne
    Recombinant
    Activité biologique
    Active
    Origine
    • 3
    • 2
    • 2
    Streptococcus pyogenes
    Source
    • 4
    • 2
    • 1
    Escherichia coli (E. coli)
    Application
    ELISA, Western Blotting (WB), SDS-PAGE (SDS)
    Attributs du produit
    Measurement of the activity: Definition of 1HU is activation of 50 % hemolysis using 3 % sheep red blood cells.
    The product was highly purified from E.coli over-expressing SLO of Group C hemolytic streptococci. The specific activity is as high as 1,900,000 hemolytic units (HU) /mg and the product forms a big hole on the cell membrane, which enables the introduction of protein inside the cells in vivo.
    Pureté
    > 98 % by SDS-PAGE
    Stérilité
    Sterile filtered
  • Indications d'application
    1) Antigen for the measurement of anti-streptolysin O antibody (ASO) (diagnostic reagent
    2) Reagent for membrane pore formation to introduce small-to-macromolecules into living cells
    Restrictions
    For Research Use only
  • Format
    Liquid
    Reconstitution
    Inactivated SLO can be reactivated by thiol reagents such as 20 mM cysteine or 10 mM DTT (2)
    Concentration
    1 mg/mL
    Buffer
    PBS, 1 mM DTT, 50 % glycerol
    Agent conservateur
    Dithiothreitol (DTT)
    Précaution d'utilisation
    This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    -20 °C/-80 °C
    Stockage commentaire
    -20 C (For long term storage: -70 C)
  • Maeda, Ide, Koike, Uchiyama, Kinoshita: "GPHR is a novel anion channel critical for acidification and functions of the Golgi apparatus." dans: Nature cell biology, Vol. 10, Issue 10, pp. 1135-45, (2008) (PubMed).

    Walev, Bhakdi, Hofmann, Djonder, Valeva, Aktories, Bhakdi: "Delivery of proteins into living cells by reversible membrane permeabilization with streptolysin-O." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 6, pp. 3185-90, (2001) (PubMed).

    Palmer: "The family of thiol-activated, cholesterol-binding cytolysins." dans: Toxicon : official journal of the International Society on Toxinology, Vol. 39, Issue 11, pp. 1681-9, (2001) (PubMed).

  • Antigène
    Streptolysin O (SLO)
    Autre désignation
    Streptolysin O (SLO Produits)
    Synonymes
    streptolysin O Protein, Streptolysin O Protein, Thiol-activated cytolysin Protein, slo Protein, M6_Spy0188 Protein
    Sujet
    Streptolysin O (SLO) is a membrane-damaging extracellular toxin produced by hemolytic streptococci. The membrane-damaging activity is measured by hemolysis of red-blood cells. SLO is easily inactivated in the presence of oxygen but can be reactivated by thiol compounds, so it is also called thiol-activated cytolysin. SLO is produced not only by Group A hemolytic streptococci but also by Group C and Group G strains. The amino acid sequences are highly conserved among them and their homology is over 98 %.
Vous êtes ici:
Support technique