The absence of endonucleases and exonucleases was confirmed.
Pureté
> 95 % of protein determined by SDS-PAGE (CBB staining)
Indications d'application
1. Promoting DNA replication and recombination by stabilizing single-stranded DNA 2. Increase specificity and yields of long PCR products
Restrictions
For Research Use only
Format
Liquid
Concentration
10 mg/mL
Buffer
20 mM Tris-HCl ( pH 8.0), 100 mM NaCl, 0.5 mM dithiothreitol, 1 mM EDTA, 50 % glycerol
Agent conservateur
Dithiothreitol (DTT)
Précaution d'utilisation
This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
-20 °C
Bittner, Burke, Alberts: "Purification of the T4 gene 32 protein free from detectable deoxyribonuclease activities." dans: The Journal of biological chemistry, Vol. 254, Issue 19, pp. 9565-72, (1979) (PubMed).
Alberts, Frey: "T4 bacteriophage gene 32: a structural protein in the replication and recombination of DNA." dans: Nature, Vol. 227, Issue 5265, pp. 1313-8, (1970) (PubMed).
Antigène
T4 SSB
Classe de substances
Phage Protein
Sujet
T4 gene 32 protein is a single-stranded DNA binding protein from phage T4 which binds to single-stranded DNA with high specificity. It is involved in DNA replication and recombination. The T4 phage-derived SSB gene was expressed in E.coli and the protein was highly purified. MW is 33.5 kDa.