Taq SSB (Active) Protéine
Origine: Thermus aquaticus
Hôte: Escherichia coli (E. coli)
Recombinant
> 95 % of protein determined by SDS-PAGE (CBB staining)
Func
Active
(1 reference)-
- Antigène
- Taq SSB
- Type de proteíne
- Recombinant
- Activité biologique
- Active
- Origine
- Thermus aquaticus
- Source
- Escherichia coli (E. coli)
- Application
- Functional Studies (Func)
- Attributs du produit
- The absence of endonucleases and exonucleases was confirmed.
- Pureté
- > 95 % of protein determined by SDS-PAGE (CBB staining)
-
- Indications d'application
- Stabilizes single-stranded DNA in DNA replication,repair,and recombination
- Commentaires
-
Activity: Single-stranded DNA binding activity was confirmed.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
- 50 mM Tris-HCl ( pH 8. 0), 200 mM NaCl, 0. 1 mM dithiothreitol, 0. 5 mM EDTA, 50 % glycerol
- Agent conservateur
- Dithiothreitol (DTT)
- Précaution d'utilisation
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- -20 °C
-
-
: "Novel thermostable ssDNA-binding proteins from Thermus thermophilus and T. aquaticus-expression and purification." dans: Protein expression and purification, Vol. 26, Issue 1, pp. 131-8, (2002) (PubMed).
-
: "Novel thermostable ssDNA-binding proteins from Thermus thermophilus and T. aquaticus-expression and purification." dans: Protein expression and purification, Vol. 26, Issue 1, pp. 131-8, (2002) (PubMed).
-
- Antigène
- Taq SSB
- Sujet
- Thermus aquaticus derived single-stranded DNA binding protein (SSB) is a thermostable protein which binds to single-stranded DNA with high specificity but does not bind well to double-stranded DNA. It plays important roles in DNA replication and recombination. Thermus aquaticus SSB gene was expressed in E.Coli in large quantities and the protein was highly purified. MW is 30.0 kDa, same as that of the natural protein.
- UniProt
- Q9KH06
-
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