Biological activity was determined by a cell proliferation assay using balb/c 3T3 cells. The expected ED50 is < 0.1 ng/mL, in the presence of 10 μg/mL heparin, corresponding to a specific activity of > 1 x 107 units/mg.
Pureté
< 95 % by SDS-PAGE gel and HPLC analyses.
niveau d'endotoxine
Endotoxin level is less than 0.1 ng per μg (1 EU/μg).
Origine: Humain
Hôte: Escherichia coli (E. coli)
Recombinant
>98 % as determined by SDS-PAGE.
Restrictions
For Research Use only
Format
Lyophilized
Conseil sur la manipulation
As with any protein, exposing FGF-acidic recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Stock
-20 °C
Stockage commentaire
The recombinant protein is stable for at least 2 years from date of receipt at -20 °C. Reconstituted FGF-acidic stable for at least 3 months when stored in working aliquots with a carrier protein at -20 °C.
Date de péremption
24 months
Antigène
FGF acidic
Autre désignation
FGF-Acidic
Sujet
FGF-acidic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-acidic is a non-glycosylated heparin binding growth factor that is expressed in the brain, kidney, retina, smooth muscle cells, bone matrix, osteoblasts, astrocytes and endothelial cells. FGF-acidic has the ability to signal through all the FGF receptors. Recombinant rat FGF-acidic is a 15.9 kDa protein consisting of 141 amino acid residues.