COL18A1 Protein (AA 1591-1774)
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- Antigène Voir toutes COL18A1 Protéines
- COL18A1 (Collagen, Type XVIII, alpha 1 (COL18A1))
- Type de proteíne
- Recombinant
- Activité biologique
- Active
- Attributs du protein
- AA 1591-1774
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Origine
- Souris
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Source
- HEK-293 Cells
- Application
- Western Blotting (WB), Immunohistochemistry (IHC)
- Pureté
- > 95 % , as determined by Coomassie stained SDS-PAGE.
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
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Less than 0.01 ng per μg cytokine as determined by the LAL method.
- Top Product
- Discover our top product COL18A1 Protéine
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Commentaires
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Biological activity: ED50 = 1.0 - 6.0 μg /mL, as determined by the inhibition of HUVEC cell proliferation, which is induced by human FGF basic in the presence of 2 μg/ml of fibronectin.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
- For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20 °C to -70 °C. Stock solutions can also be prepared at 50 - 100 μg/mL in sterile buffers (PBS, HPBS, DPBS, or EBSS) containing carrier proteins such as 0.2-1 % BSA or HAS, and stored in working aliquots at -20 °C to -70 °C.
- Buffer
- 0.22 μm filtered protein solution is in 10 mM NaHPO4, 0.3 M NaCl, and pH 7.2.
- Conseil sur la manipulation
- Avoid repeated freeze/thaw cycles.
- Stock
- -20 °C
- Stockage commentaire
- Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.
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- Antigène
- COL18A1 (Collagen, Type XVIII, alpha 1 (COL18A1))
- Autre désignation
- Endostatin (COL18A1 Produits)
- Synonymes
- KNO Protein, KNO1 Protein, KS Protein, cb371 Protein, cb529 Protein, fc96d06 Protein, sb:cb529 Protein, wu:fb47h10 Protein, wu:fb77g12 Protein, wu:fc50f04 Protein, wu:fc96d06 Protein, wu:fk02h12 Protein, kno Protein, kno1 Protein, collagen type XVIII alpha 1 chain Protein, collagen type XVIII alpha 1 chain a Protein, collagen, type XVIII, alpha 1 Protein, collagen type XVIII alpha 1 Protein, COL18A1 Protein, col18a1a Protein, Col18a1 Protein, col18a1 Protein
- Classe de substances
- Chemical
- Sujet
- Endostatin is a C-terminal proteolytic fragment of collagen XVIII α1 (Col 18) that is localized in the vascular basement membrane of almost all epithelia and endothelia. Endostatin is produced from catalytic cleavage from MMPs, cathepsin L, and S. It is detected in circulation at a physiologic level of 20 to 50 ng/mL in serum. Crystal X-ray analysis of endostatin has shown a compact globular structure, with one face rich in arginine residues that acts as a heparin-binding motif. Endostatin interacts with multiple molecules including heparin, heparan sulfate, chondroitin sulfate, dermatan sulfate, thrombospondin-1, SPARC, collagens (I, IV, and VI), the amyloid peptide Aβ (1-42), transglutaminase-2, and among others. Endostatins derived from collagens XV and XVIII differ in structure and binding properties, tissue distribution, and anti-angiogenic activity. Endostatin is an endogenous angiostatic factor that induces apoptosis in endothelial cells and inhibits endothelial cell migration in vitro. Col 18-null mice showed faster tumor growth when implanted with tumor cells that do not produce Col 18. Overexpression of circulating endostatin in transgenic mice resulted in reduced tumor growth. Experimental model of glomerulonephritis in Col 18-null mice showed that the absence of this molecule exacerbates immune-mediated glomerulonephritis.
- Poids moléculaire
- The 192 amino acid recombinant protein has a predicted molecular mass of approximately 21.3 kDa. The DTT-reduced and non-reduced protein migrate at approximately 23 and 20 kDa respectively by SDS-PAGE. The predicted N-terminal amino acid is His.
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