IL-25 Protein (AA 33-177, N-Term)
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- Antigène Voir toutes IL-25 (IL25) Protéines
- IL-25 (IL25) (Interleukin 25 (IL25))
- Type de proteíne
- Recombinant
- Activité biologique
- Active
- Attributs du protein
- AA 33-177, N-Term
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Origine
- Humain
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Source
- Escherichia coli (E. coli)
- Application
- Western Blotting (WB)
- Pureté
- >98 % , as determined by Coomassie stained SDS-PAGE.
- niveau d'endotoxine
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Less than 0.1 ng per μg of protein.
- Top Product
- Discover our top product IL25 Protéine
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Commentaires
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Biological activity: Human IL-17E is able to induce IL-8 secretion in human PBMCs using a concentration range of 10 - 100 ng/ml. Note: Results will vary with PBMC donors.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. It is recommended to further dilute in a buffer containing a carrier protein such as 0.1 % BSA and store working aliquots at -20 °C to -80 °C.
- Buffer
- Lyophilized
- Conseil sur la manipulation
- Avoid repeated freeze/thaw cycles.
- Stock
- -20 °C
- Stockage commentaire
- Unopened vial can be stored at -20°C or -70°C.
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- Antigène
- IL-25 (IL25) (Interleukin 25 (IL25))
- Autre désignation
- IL-17E (IL25 Produits)
- Synonymes
- IL17E Protein, IL-17e Protein, Il17e Protein, IL25 Protein, RGD1561632 Protein, interleukin 25 Protein, IL25 Protein, Il25 Protein
- Sujet
- IL-17E, also known as IL-25, promotes Th2 biased immune responses. This is in contrast to other IL-17 family members which promote Th1 and Th17 biased inflammation. IL-17E is an important mediator of allergic reactions and protection against intestinal parasites. During helminth infections and allergic reactions, IL-17E production is locally upregulated in intestinal cells, airway epithelial cells, atopic dermatitis skin lesions, local Th2 cells, eosinophils, and basophils. It binds to IL-17RB but also requires IL-17RA to exert its activity. IL-17E acts on a variety of cell types which respond with increased production of Th2 cytokines and reduced production of Th1 and Th17 cytokines. Airway IL-17E can be activated by MMP7, a protease that is upregulated in airway epithelium in response to allergen exposure. Cleaved IL-17E shows enhanced binding to IL-17RB and stronger induction of Th2 cytokines. The Th2 cytokines, in turn, trigger the expansion of Th2 memory cells and anti-inflammatory M2 macrophages, increased eosinophil mobilization and activation, and dendritic cell migration. These actions promote protective anti-helminth immune responses as well as allergic inflammation and airway hyperreactivity. IL-17E also promotes vascular endothelial cell proliferation and assembly into tubular structures. It also supports the integrity of the blood brain barrier and limits CD4+ T cell infiltration into the brain.
- Poids moléculaire
- The 146 amino acid recombinant protein has a predicted molecular mass of approximately 17 kDa. Recombinant human IL-17E forms a non-disulfide-linked homodimer (34 kDa). The predicted N-terminal amino acid is Met.
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