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IL-27 Protein (AA 1-16, AA 29-243, Arg21)

IL27 Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant >95 % , as determined by Coomassie stained SDS-PAGE. FACS Active
N° du produit ABIN2667515
  • Antigène Voir toutes IL-27 (IL27) Protéines
    IL-27 (IL27) (Interleukin 27 (IL27))
    Type de proteíne
    Recombinant
    Activité biologique
    Active
    Attributs du protein
    AA 1-16, Arg21, AA 29-243
    Origine
    • 7
    • 7
    • 1
    Humain
    Source
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    Escherichia coli (E. coli)
    Application
    Flow Cytometry (FACS)
    Pureté
    >95 % , as determined by Coomassie stained SDS-PAGE.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine

    Less than 0.01 ng per μg cytokine as determined by the LAL method.

    Top Product
    Discover our top product IL27 Protéine
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Commentaires

    Biological activity: ED50 = 30 - 150 ng/ml, corresponding to a specific activity of 0.66 - 3.3 x 104 units/mg, as determined by mouse splenocytes IL-2 inhibition induced by hIL-27 in mouse splenocytes activated with anti-CD3 and anti-CD28 antibodies.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Reconstitution
    For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.
    Buffer
    0.22 μm filtered protein solution is in 20 mM NaHPO4, pH 6, 0.60 M NaCl.
    Conseil sur la manipulation
    Avoid repeated freeze/thaw cycles.
    Stock
    -20 °C
    Stockage commentaire
    Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.
  • Antigène
    IL-27 (IL27) (Interleukin 27 (IL27))
    Autre désignation
    IL-27 (IL27 Produits)
    Synonymes
    IL-27 Protein, IL-27A Protein, IL27A Protein, IL27p28 Protein, IL30 Protein, p28 Protein, RGD1561420 Protein, IL27 Protein, IL-27p28 Protein, Il30 Protein, interleukin 27 Protein, IL27 Protein, Il27 Protein
    Sujet
    IL-27 is a heterodimeric cytokine that consists of EBI3, an IL-12p40-related protein, and p28, an IL-12p35-related polypeptide. IL-27 can be allocated to the IL-6/IL-12 superfamily of cytokines which also includes IL-23. This family is defined based on similarities in the structural motifs of the ligands, such as a common four-helix bundle and their receptors, which contain a hematopoietin receptor domain. IL-27 signaling occurs via a receptor complex composed of the signal transducing receptor chains WSX-1 and glycoprotein (gp) 130. Whereas WSX-1 is the IL-27-specific receptor chain, gp130 is the common receptor subunit of IL-6 type cytokines. IL-27 is expressed at sites of inflammation in cytokine-driven autoimmune/inflammatory diseases, such as rheumatoid arthritis, psoriasis, inflammatory bowel disease, and sarcoidosis.
    Poids moléculaire
    The total predicted molecular weight is 50 kDa. The non-reduced and the DTT-reduced proteins migrate as one band at approximately 60 kDa by SDS-PAGE.
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