MYC
Origine: Humain
Hôte: Escherichia coli (E. coli)
Recombinant
The purified recombinant protein is greater than 75 % homogeneous and contains no detectable protease, DNase and RNase activity.
PI
Recombinant c-Myc was expressed in a bacterial system (accession number NM 002467) with an amino terminal polyhistidine tag and purified by an affinity column in combination with FPLC chromatography. The purified recombinant protein is greater than 75 % homogeneous and contains no detectable protease, DNase and RNase activity.
Purification
Purified by an affinity column in combination with FPLC chromatography.
Pureté
The purified recombinant protein is greater than 75 % homogeneous and contains no detectable protease, DNase and RNase activity.
Recombinant c-Myc protein is suitable for DNA and protein-protein interaction assays. 100 ng are sufficient for DNA-protein and protein-protein interaction studies. The standard curve for TransAM® c-Myc was generated using the range of 5-0.625 ng of protein. The molecular weight of the protein is ~64 kDa. NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and should be avoided.
C-Myc (Myc) is a sequence specific DNA binding transcription factor that regulates gene expression, especially genes involved in cell division. Myc family members contain a basic Helix-Loop-Helix leucine zipper (bHLHZ) domain. Myc family proteins associated with thousands of genes, especially those involved in cell growth and proliferation. c-Myc serves as an integrator of signal transduction, linking extracellular signals to an acute cellular growth response. Mutations and overexpression of c-Myc are associated with cell proliferation and cancer.