NR1H4
Origine: Humain
Hôte: Escherichia coli (E. coli)
Recombinant
The purified recombinant protein has an amino terminal polyhistidine tag and is greater than 90 % homogeneous and contains no detectable protease, DNase and RNase activity.
PI
NR1H4
(Nuclear Receptor Subfamily 1, Group H, Member 4 (NR1H4))
Type de proteíne
Recombinant
Origine
Humain
Source
Escherichia coli (E. coli)
Purification/Conjugué
Cette NR1H4 protéine est marqué à la His tag.
Application
Protein Interaction (PI)
Attributs du produit
Recombinant FXR is isolated from an E. coli strain that carries the coding sequence of the human FXR under the control of a T7 promoter (accession number U68233). The purified recombinant protein has an amino terminal polyhistidine tag and is greater than 90 % homogeneous and contains no detectable protease, DNase and RNase activity.
Pureté
The purified recombinant protein has an amino terminal polyhistidine tag and is greater than 90 % homogeneous and contains no detectable protease, DNase and RNase activity.
NR1H4
Origine: Rat
Hôte: Escherichia coli (E. coli)
Recombinant
WB, SDS, ELISA, IP
Indications d'application
Recombinant FXR is suitable for DNA and protein-protein interaction assays. 20 ng is sufficient for gelshift assays and 100 ng is sufficient for protein-protein interaction studies. The molecular weight of the protein is ~55 kDa. NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and should be avoided.
Restrictions
For Research Use only
Concentration
0.1 μg/μL
Antigène
NR1H4
(Nuclear Receptor Subfamily 1, Group H, Member 4 (NR1H4))
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Sujet
The farnesoid X receptor (FXR) or nuclear receptor subfamily 1, group H, member 4 (NR1H4) is a nuclear receptor that, when activated, translocates to the nucleus where it interacts with certain proteins, including RXR and PPARGC1A, forming heterodimers that bind to hormone response elements on DNA and modulate gene expression. One of the primary functions of FXR is to suppress bile acid synthesis when levels are high by inhibiting the transcription of cholesterol 7 alpha-hydroxylase (CYP7A1) gene that expresses CYP7A1, the rate-limiting enzyme in bile acid synthesis from cholesterol.