Histone H3.3 Protein (biotinylated, C-Term, full length)

N° du produit ABIN2669583

Détail du produit

Antigène: Histone H3.3

Type de proteíne: Recombinant

Attributs du protein: C-Term, biotinylated, full length

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Enzyme Activity Assay (EAA), Screening Assay (ScA)

Attributs du produit: Recombinant Histone H3.3 - biotinylated, Human, corresponds to the native histone sequence of mammalian histone H3 variant H3.3 and does not contain any amino acid substitutions or residue analogs. Histone H3.3 is known to be important for gene regulation. Recombinant Histone H3.3 - biotinylated, Human consists of the full-length sequence of human Histone H3.3 variant (accession number NM_005324) and contains a C-terminal Biotin-tag. Recombinant Histone H3.3 - biotinylated, Human was generated in E. coli cells and has an observed molecular weight of 18 kDa.

Information d'application

Indications d'application: Recombinant Histone H3.3 - biotinylated, Human is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

Restrictions: For Research Use only

Stockage

Concentration: 0.4 μg/μL

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Stock: -80 °C

Stockage commentaire: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.

Détail du antigène

Antigène: Histone H3.3

Abstract: Histone H3.3 Produits

Synonymes: H3.3A Protein, H3F3 Protein, H3-IX Protein, H3-X Protein, H3.3B Protein, H3F3C Protein, H3F3B Protein, H3 histone family member 3A Protein, H3 histone, family 3A Protein, H3 histone, family 3B Protein, H3 histone, family 3C L homeolog Protein, histone H3.3 Protein, Histone H3.3 Protein, histone H3.3B-like Protein, H3F3A Protein, H3F3B Protein, H3f3a Protein, h3f3c.L Protein, LOC5567559 Protein, Bm1_29875 Protein, Bm1_40030 Protein, PTRG_03457 Protein, Phum_PHUM492720 Protein, UREG_07214 Protein, PAAG_07099 Protein, PITG_06950 Protein, PITG_06953 Protein, PITG_06955 Protein, LOC100361558 Protein, LOC100521680 Protein

Sujet: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker protein, present at the interface between the nucleosome core and DNA entry/exit points. Histone H3.1 and Histone H3.3 are the two main Histone H3 variants found in plants and animals. They are known to be important for gene regulation. Histone H3.1 and H3.3 have been shown to demonstrate unique genomic localization patterns thought to be associated with their specific functions in regulation of gene activity. Specifically, Histone H3.1 localization is found to coincide with genomic regions containing chromatin repressive marks (H3K9me3, H3K27me3 and DNA methylation), whereas Histone H3.3 primarily colocalizes with marks associated with gene activation (H3K4me3, H2BK120ub1, and RNA pol II occupancy). Deposition of the Histone H3.1 variant into the nucleosome correlates with the canonical DNA synthesis-dependent deposition pathway, whereas Histone H3.3 primarily serves as the replacement Histone H3 variant outside of S-phase, such as during gene transcription. Aberrant localization of these variants is also known to correlate with certain cancers.

Poids moléculaire: 18 kDa