SETD2 Protein (DYKDDDDK Tag)

N° du produit ABIN2669663

Détail du produit

Antigène: SETD2 (SET Domain Containing 2 (SETD2))

Type de proteíne: Recombinant

Origine: Humain

Source: Baculovirus

Purification/Conjugué: Cette SETD2 protéine est marqué à la DYKDDDDK Tag.

Application: Enzyme Activity Assay (EAA), Screening Assay (ScA)

Attributs du produit: Recombinant SETD2 (1392-2564) (accession number NP_054878.5) was expressed in Sf9 cells and contains an N-terminal FLAG tag with an observed molecular weight of 134 kDa.

Information d'application

Indications d'application: Recombinant SETD2 (1392-2564) is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3K36 methyltransferase. HMT Assay Conditions: 50 mM TrisCl, pH 8.6, 0.02 % Triton X-100, 2 mM MgCl2, 1 mM TCEP, 100 μM SAM, 30 ng/μl Recombinant Nucleosomes, 30 ng/μl Recombinant SETD2 (1392-2564) at 2 hours at room temperature. Activity was detected by fluorography.

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Stock: -80 °C

Stockage commentaire: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.

Détail du antigène

Antigène: SETD2 (SET Domain Containing 2 (SETD2))

Autre désignation: SETD2 (SETD2 Produits)

Synonymes: Kif9 Protein, HBP231 Protein, HIF-1 Protein, HIP-1 Protein, HYPB Protein, KMT3A Protein, SET2 Protein, p231HBP Protein, SET domain containing 2 Protein, Setd2 Protein, SETD2 Protein

Sujet: SETD2 (SET Domain Containing 2) is a histone methyltransferase that methylates histone H3 at lysine 36. H3K36 methylation is associated with active chromatin. SETD2 also contains a novel transcriptional activation domain and has been found associated with hyperphosphorylated RNA pol II. Recombinant SETD2 (1392-2564) includes amino acids 1392-2564 that contains the SET domain of SETD2 that catalyzes methylation of lysine residues.

Poids moléculaire: 134 kDa

Pathways: Tube Formation