E2F1 Protein (Myc-DYKDDDDK Tag)

N° du produit ABIN2719894

Détail du produit

Antigène: E2F1 (E2F Transcription Factor 1 (E2F1))

Type de proteíne: Recombinant

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette E2F1 protéine est marqué à la Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Attributs du produit:

• Recombinant human E2F1 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Pureté: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Information d'application

Indications d'application: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Commentaires:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Stockage

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Stock: -80 °C

Stockage commentaire: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Référence

Tarangelo, Lo, Teng, Kim, Le, Watson, Furth, Raman, Ehmer, Viatour: "Recruitment of Pontin/Reptin by E2f1 amplifies E2f transcriptional response during cancer progression." dans: Nature communications, Vol. 6, pp. 10028, (2015) (PubMed).

Détail du antigène

Antigène: E2F1 (E2F Transcription Factor 1 (E2F1))

Autre désignation: e2f1 (E2F1 Produits)

Synonymes: CG6376 Protein, DRTF1/E2F Protein, DmE2F-1 Protein, Dmel\\CG6376 Protein, Dp Protein, E(Sev-CycE)3A Protein, E(var)3-93E Protein, E(var)3-95E Protein, E(var)93E Protein, E2F Protein, E2F-1 Protein, E2F1 Protein, E2f1 Protein, Evar(3)164 Protein, dE2F Protein, dE2F1 Protein, dE2f Protein, dE2f1 Protein, de2f1 Protein, def21 Protein, drosE2F1 Protein, e2f Protein, e2f1 Protein, l(3)07172 Protein, l(3)j3B1 Protein, l(3)j3C2 Protein, l(3)rM729 Protein, RBAP1 Protein, RBBP3 Protein, RBP3 Protein, mKIAA4009 Protein, xE2F Protein, ATE2FB Protein, E2F TRANSCRIPTION FACTOR-1 E2F1 Protein, E2F transcription factor 1 Protein, E2FB Protein, E2FB TRANSCRIPTION FACTOR Protein, E2F transcription factor 1 Protein, E2F transcription factor 1 L homeolog Protein, E2f1 Protein, E2F1 Protein, e2f1.L Protein

Sujet: The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.

Poids moléculaire: 46.7 kDa

NCBI Accession: NP_005216

Pathways: Signalisation p53, Cycle Cellulaire, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy