Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)
Specificité
Optimal preservation of protein structure, post-translational modifications and functions.
Attributs du produit
Recombinant human Interleukin-27 / IL27A protein expressed in HEK293.
Produced with end-sequenced ORF clone
Tested for bioactivity.
Pureté
Purity is > 95 % , as determined by Coomassie stained SDS-PAGE.
niveau d'endotoxine
Less than 0.01 ng per μg protein as determined by the LAL method.
Biological Activity Comment
The ED50 is 5 - 10 ng/ml, corresponding to a specific activity of 1.0 - 2.0 x 10^5 units/mg, determined by inhibition of IL-2 production induced by mouse IL-27 in mouse splenocytes activated with anti-CD3 and anti-CD28 antibodies.
IL27
Origine: Rat
Hôte: Escherichia coli (E. coli)
Recombinant
> 95 %
WB, Imm, PC, SDS
Indications d'application
Recombinant human proteins can be used for: Native antigens for optimized antibody production Positive controls in ELISA and other antibody assays Protein-protein interaction In vitro biochemical assays and cell-based functional assays
Restrictions
For Research Use only
Concentration
> 50 μg/mL
Buffer
20 mM NaH2PO4, pH 6, 0.60 M NaCl
Stock
-80 °C
Stockage commentaire
Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.