KPNA1 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)
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- Antigène Voir toutes KPNA1 Protéines
- KPNA1 (Karyopherin alpha 1 (Importin alpha 5) (KPNA1))
- Type de proteíne
- Recombinant
- Attributs du protein
- Transcript Variant 1
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Origine
- Humain
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Source
- HEK-293 Cells
- Purification/Conjugué
- Cette KPNA1 protéine est marqué à la Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Attributs du produit
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- Recombinant human KPNA1 / Importin alpha-1 (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Pureté
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product KPNA1 Protéine
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- Indications d'application
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Commentaires
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Antigène
- KPNA1 (Karyopherin alpha 1 (Importin alpha 5) (KPNA1))
- Autre désignation
- Kpna1,importin alpha-1 (KPNA1 Produits)
- Synonymes
- IPOA5 Protein, NPI-1 Protein, RCH2 Protein, SRP1 Protein, AW494490 Protein, NPI1 Protein, Rch2 Protein, mSRP1 Protein, si:ch211-161h7.7 Protein, karyopherin subunit alpha 1 Protein, importin alpha-1 subunit Protein, karyopherin (importin) alpha 1 Protein, karyopherin alpha 1 (importin alpha 5) Protein, KPNA1 Protein, Kpna1 Protein, EHI_124900 Protein, kpna1 Protein
- Sujet
- Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. [UniProtKB/Swiss-Prot Function]
- Poids moléculaire
- 60 kDa
- NCBI Accession
- NP_002255
- Pathways
- M Phase, Protein targeting to Nucleus
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