Neurotrophin 3 Protein (NTF3) (Transcript Variant 2)
NTF3
Origine: Humain
Hôte: Escherichia coli (E. coli)
Recombinant
> 95 % as determined by SDS-PAGE and Coomassie blue staining
Func, AbP, STD, PI
Active
Functional Studies (Func), Antibody Production (AbP), Standard (STD), Protein Interaction (PI)
Specificité
Optimal preservation of protein structure, post-translational modifications and functions.
Attributs du produit
Recombinant human Neurotrophin 3 / NTF3 (transcript variant 2) protein expressed in E. coli.
Produced with end-sequenced ORF clone
Tested for bioactivity.
Pureté
> 95 % as determined by SDS-PAGE and Coomassie blue staining
niveau d'endotoxine
Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
Biological Activity Comment
The ED50 as determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cell cultures was found to be in the range of 20-50 ng/ml.
NTF3
Origine: Boeuf (Vache)
Hôte: Escherichia coli (E. coli)
Recombinant
> 97 %
WB, SDS, Imm, PC
Indications d'application
Recombinant human proteins can be used for: Native antigens for optimized antibody production Positive controls in ELISA and other antibody assays Protein-protein interaction In vitro biochemical assays and cell-based functional assays
Restrictions
For Research Use only
Buffer
Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
Conseil sur la manipulation
Resuspend the protein in the desired concentration in proper buffer
Stock
-80 °C
Stockage commentaire
Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.