Nucleolin Protein (NCL) (Myc-DYKDDDDK Tag)

N° du produit ABIN2727708

Détail du produit

Antigène: Nucleolin (NCL)

Type de proteíne: Recombinant

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette Nucleolin protéine est marqué à la Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Attributs du produit:

• Recombinant human Nucleolin protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Pureté: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Information d'application

Indications d'application: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Commentaires:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Stockage

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Stock: -80 °C

Stockage commentaire: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Référence

Hernández, Sandoval-Jaime, Sosnovtsev, Green, Gutiérrez-Escolano: "Nucleolin promotes in vitro translation of feline calicivirus genomic RNA." dans: Virology, Vol. 489, pp. 51-62, (2016) (PubMed).

Perrone, Butovskaya, Lago, Garzino-Demo, Pannecouque, Palù, Richter: "The G-quadruplex-forming aptamer AS1411 potently inhibits HIV-1 attachment to the host cell." dans: International journal of antimicrobial agents, Vol. 47, Issue 4, pp. 311-6, (2016) (PubMed).

Tosoni, Frasson, Scalabrin, Perrone, Butovskaya, Nadai, Palù, Fabris, Richter: "Nucleolin stabilizes G-quadruplex structures folded by the LTR promoter and silences HIV-1 viral transcription." dans: Nucleic acids research, Vol. 43, Issue 18, pp. 8884-97, (2015) (PubMed).

Détail du antigène

Antigène: Nucleolin (NCL)

Autre désignation: Nucleolin (NCL Produits)

Synonymes: C23 Protein, B530004O11Rik Protein, D0Nds28 Protein, D1Nds28 Protein, Nucl Protein, wu:fa12d03 Protein, zgc:152810 Protein, nucl Protein, LOC100282923 Protein, LOC100283058 Protein, nucleolin Protein, nucleolin S homeolog Protein, NCL Protein, Ncl Protein, ncl.S Protein, ncl Protein, LOC100195537 Protein, LOC100282923 Protein, LOC100283058 Protein

Sujet: Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.

Poids moléculaire: 76.4 kDa

NCBI Accession: NP_005372

Pathways: Ribonucleoprotein Complex Subunit Organization