TNF alpha
Origine: Humain
Hôte: Escherichia coli (E. coli)
Recombinant
Purity is > 98 % , as determined by Coomassie stained SDS-PAGE.
Func, STD, AbP, PI
Active
Functional Studies (Func), Standard (STD), Antibody Production (AbP), Protein Interaction (PI)
Specificité
Optimal preservation of protein structure, post-translational modifications and functions.
Attributs du produit
Recombinant human Tumor necrosis factor (TNF-alpha) protein expressed in E. coli.
Produced with end-sequenced ORF clone
Tested for bioactivity.
Pureté
Purity is > 98 % , as determined by Coomassie stained SDS-PAGE.
niveau d'endotoxine
Less than 0.01 ng per ug protein as determined by the LAL method.
Biological Activity Comment
The ED50 is 0.020-0.050 ng/ml, corresponding to a specific activity of 5-2 X10^7 units/mg, determined by a dose dependent stimulation of L929 cells treated with actinomycin D.
TNF alpha
Origine: Humain
Hôte: HEK-293 Cells
Recombinant
The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
TNF alpha
Origine: Humain
Hôte: Yeast (Pichia pastoris)
> 97 % by SDS-PAGE.
Active
Indications d'application
Recombinant human proteins can be used for: Native antigens for optimized antibody production Positive controls in ELISA and other antibody assays Protein-protein interaction In vitro biochemical assays and cell-based functional assays
Restrictions
For Research Use only
Concentration
> 50 μg/mL
Buffer
10 mM NaH2PO4, 150 mM NaCl, pH 7.2
Stock
-80 °C
Stockage commentaire
Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.