TWSG1
Origine: Humain
Hôte: Escherichia coli (E. coli)
Recombinant
> 95 % as determined by SDS-PAGE and Coomassie blue staining
AbP, STD, Func, PI
Active
Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)
Specificité
Optimal preservation of protein structure, post-translational modifications and functions.
Attributs du produit
Recombinant human TWSG1 / Tsg protein expressed in E. coli.
Produced with end-sequenced ORF clone
Tested for bioactivity.
Pureté
> 95 % as determined by SDS-PAGE and Coomassie blue staining
niveau d'endotoxine
Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
Biological Activity Comment
Determined by its ability to neutralize BMP-6 induced alkaline phosphatase production by ATDC chondrogenic cells. The expected ED50 for this effect is > 2.0 ug/mL of TSG.
Recombinant human proteins can be used for: Native antigens for optimized antibody production Positive controls in ELISA and other antibody assays Protein-protein interaction In vitro biochemical assays and cell-based functional assays
Restrictions
For Research Use only
Buffer
Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
Conseil sur la manipulation
Resuspend the protein in the desired concentration in proper buffer
Stock
-80 °C
Stockage commentaire
Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.