UMPS Protein (Myc-DYKDDDDK Tag)
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- Antigène Voir toutes UMPS Protéines
- UMPS (Uridine Monophosphate Synthetase (UMPS))
- Type de proteíne
- Recombinant
- Activité biologique
- Active
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Origine
- Humain
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Source
- HEK-293 Cells
- Purification/Conjugué
- Cette UMPS protéine est marqué à la Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)
- Specificité
- Optimal preservation of protein structure, post-translational modifications and functions.
- Attributs du produit
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- Recombinant human UMPS protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- Pureté
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Biological Activity Comment
- UMPS activity is verified in a bioassay:
- Top Product
- Discover our top product UMPS Protéine
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- Indications d'application
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - Commentaires
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- > 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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Metabolomics identifies pyrimidine starvation as the mechanism of 5-aminoimidazole-4-carboxamide-1-β-riboside-induced apoptosis in multiple myeloma cells." dans: Molecular cancer therapeutics, Vol. 12, Issue 7, pp. 1310-21, (2013) (PubMed).
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Metabolomics identifies pyrimidine starvation as the mechanism of 5-aminoimidazole-4-carboxamide-1-β-riboside-induced apoptosis in multiple myeloma cells." dans: Molecular cancer therapeutics, Vol. 12, Issue 7, pp. 1310-21, (2013) (PubMed).
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- Antigène
- UMPS (Uridine Monophosphate Synthetase (UMPS))
- Autre désignation
- Umps (UMPS Produits)
- Synonymes
- zgc:55702 Protein, zgc:55723 Protein, UMPS Protein, OPRT Protein, 1700095D23Rik Protein, AA408257 Protein, AL033308 Protein, BB164745 Protein, Orotidine 5'-phosphate decarboxylase Protein, uridine monophosphate synthetase Protein, uridine monophosphate synthetase L homeolog Protein, uridine 5'-monophosphate synthase Protein, umps-1 Protein, umps Protein, umps.L Protein, UMPS Protein, LOC100556619 Protein, Umps Protein
- Sujet
- This gene encodes a uridine 5&apos-monophosphate synthase. The encoded protein is a bifunctional enzyme that catalyzes the final two steps of the de novo pyrimidine biosynthetic pathway. The first reaction is carried out by the N-terminal enzyme orotate phosphoribosyltransferase which converts orotic acid to orotidine-5&apos-monophosphate. The terminal reaction is carried out by the C-terminal enzyme OMP decarboxylase which converts orotidine-5&apos-monophosphate to uridine monophosphate. Defects in this gene are the cause of hereditary orotic aciduria. Alternate splicing results in multiple transcript variants.
- Poids moléculaire
- 52 kDa
- NCBI Accession
- NP_000364
- Pathways
- Ribonucleoside Biosynthetic Process
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