WISP3 Protein (Transcript Variant 1)

N° du produit ABIN2735603

Détail du produit

Antigène: WISP3 (Wnt1 Inducible Signaling Pathway Protein 3 (WISP3))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: Transcript Variant 1

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Functional Studies (Func), Antibody Production (AbP), Protein Interaction (PI), Standard (STD)

Specificité: Optimal preservation of protein structure, post-translational modifications and functions.

Attributs du produit:

• Recombinant human WISP3 (transcript variant 1) protein expressed in E. coli.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Pureté: > 95 % as determined by SDS-PAGE and Coomassie blue staining

niveau d'endotoxine: Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).

Biological Activity Comment: ED50 was determined by the dose-dependant proliferation of the MCF-7 cell line. The expected ED50 for this effect is 0.2-0.3 ug/ml.

Information d'application

Indications d'application: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Restrictions: For Research Use only

Stockage

Buffer: Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2

Conseil sur la manipulation: Resuspend the protein in the desired concentration in proper buffer

Stock: -80 °C

Stockage commentaire: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Détail du antigène

Antigène: WISP3 (Wnt1 Inducible Signaling Pathway Protein 3 (WISP3))

Autre désignation: Wisp3 (WISP3 Produits)

Synonymes: CCN6 Protein, LIBC Protein, PPAC Protein, PPD Protein, ENSMUSG00000062074 Protein, Gm735 Protein, WISP-3 Protein, RGD1564120 Protein, WISP3 Protein, si:dkey-119m7.6 Protein, WNT1 inducible signaling pathway protein 3 Protein, WISP3 Protein, Wisp3 Protein, wisp3 Protein

Sujet: This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. This gene is overexpressed in colon tumors. It may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. Mutations of this gene are associated with progressive pseudorheumatoid dysplasia, an autosomal recessive skeletal disorder, indicating that the gene is essential for normal postnatal skeletal growth and cartilage homeostasis. Multiple transcript variants encoding different isoforms have been found for this gene.

Poids moléculaire: 36.8 kDa

NCBI Accession: NP_003871

Pathways: Signalisation WNT, Growth Factor Binding