GTF2F1 Protein (AA 2-517) (His tag)
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- Antigène Voir toutes GTF2F1 Protéines
- GTF2F1 (General Transcription Factor IIF, Polypeptide 1, 74kDa (GTF2F1))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 2-517
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Origine
- Humain
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Source
- Escherichia coli (E. coli)
- Purification/Conjugué
- Cette GTF2F1 protéine est marqué à la His tag.
- Application
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- Séquence
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AALGPSSQNV TEYVVRVPKN TTKKYNIMAF NAADKVNFAT WNQARLERDL SNKKIYQEEE MPESGAGSEF NRKLREEARR KKYGIVLKEF RPEDQPWLLR VNGKSGRKFK GIKKGGVTEN TSYYIFTQCP DGAFEAFPVH NWYNFTPLAR HRTLTAEEAE EEWERRNKVL NHFSIMQQRR LKDQDQDEDE EEKEKRGRRK ASELRIHDLE DDLEMSSDAS DASGEEGGRV PKAKKKAPLA KGGRKKKKKK GSDDEAFEDS DDGDFEGQEV DYMSDGSSSS QEEPESKAKA PQQEEGPKGV DEQSDSSEES EEEKPPEEDK EEEEEKKAPT PQEKKRRKDS SEESDSSEES DIDSEASSAL FMAKKKTPPK RERKPSGGSS RGNSRPGTPS AEGGSTSSTL RAAASKLEQG KRVSEMPAAK RLRLDTGPQS LSGKSTPQPP SGKTTPNSGD VQVTEDAVRR YLTRKPMTTK DLLKKFQTKK TGLSSEQTVN VLAQILKRLN PERKMINDKM HFSLKE
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Attributs du produit
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human GTF2F1 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in bacterial culture:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Endotoxin has not been removed. Please contact us if you require endotoxin removal.
- Classe de qualité
- Crystallography grade
- Top Product
- Discover our top product GTF2F1 Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- GTF2F1 (General Transcription Factor IIF, Polypeptide 1, 74kDa (GTF2F1))
- Autre désignation
- GTF2F1 (GTF2F1 Produits)
- Synonymes
- BTF4 Protein, RAP74 Protein, TF2F1 Protein, TFIIF Protein, C76800 Protein, fd13c08 Protein, rap74 Protein, wu:fd13c08 Protein, zgc:56155 Protein, AO090124000085 Protein, DDBDRAFT_0205751 Protein, DDBDRAFT_0302526 Protein, DDB_0205751 Protein, DDB_0302526 Protein, 2810405L04Rik Protein, ATRAP74 Protein, T1P17.200 Protein, T1P17_200 Protein, general transcription factor IIF subunit 1 Protein, general transcription factor IIF, polypeptide 1 Protein, transcription initiation factor IIF subunit alpha Protein, general transcription factor IIF subunit 1 L homeolog Protein, transcription initiation factor IIF subunit alpha RAP74 Protein, GTF2F1 Protein, gtf2f1 Protein, AOR_1_160104 Protein, Gtf2f1 Protein, gtf2f1.L Protein, RAP74 Protein
- Sujet
- TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation. {ECO:0000269|PubMed:10428810}.
- Poids moléculaire
- 59.1 kDa Including tag.
- UniProt
- P35269
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