TRAF1 Protein (AA 1-416) (Strep Tag)
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- Antigène Voir toutes TRAF1 Protéines
- TRAF1 (TNF Receptor-Associated Factor 1 (TRAF1))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-416
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Origine
- Humain
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Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette TRAF1 protéine est marqué à la Strep Tag.
- Application
- Western Blotting (WB), SDS-PAGE (SDS), ELISA
- Séquence
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MASSSGSSPR PAPDENEFPF GCPPTVCQDP KEPRALCCAG CLSENPRNGE DQICPKCRGE DLQSISPGSR LRTQEKAHPE VAEAGIGCPF AGVGCSFKGS PQSVQEHEVT SQTSHLNLLL GFMKQWKARL GCGLESGPMA LEQNLSDLQL QAAVEVAGDL EVDCYRAPCS ESQEELALQH FMKEKLLAEL EGKLRVFENI VAVLNKEVEA SHLALATSIH QSQLDRERIL SLEQRVVELQ QTLAQKDQAL GKLEQSLRLM EEASFDGTFL WKITNVTRRC HESACGRTVS LFSPAFYTAK YGYKLCLRLY LNGDGTGKRT HLSLFIVIMR GEYDALLPWP FRNKVTFMLL DQNNREHAID AFRPDLSSAS FQRPQSETNV ASGCPLFFPL SKLQSPKHAY VKDDTMFLKC IVETST
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
- Pureté
- > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
- Top Product
- Discover our top product TRAF1 Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- TRAF1 (TNF Receptor-Associated Factor 1 (TRAF1))
- Autre désignation
- TRAF1 (TRAF1 Produits)
- Synonymes
- CG3048 Protein, DTRAF Protein, DTRAF1 Protein, Dmel\\CG3048 Protein, TRAF Protein, TRAF1 Protein, Traf1 Protein, dTRAF Protein, dTRAF1 Protein, dTraf1 Protein, dtraf1 Protein, traf1 Protein, traf4 Protein, DKFZp469G032 Protein, EBI6 Protein, MGC:10353 Protein, 4732496E14Rik Protein, TNF receptor associated factor 1 Protein, TNF-receptor-associated factor 4 Protein, TNF receptor-associated factor 1 Protein, TRAF1 Protein, Traf4 Protein, Traf1 Protein, traf1 Protein
- Sujet
- TNF receptor-associated factor 1 (Epstein-Barr virus-induced protein 6),FUNCTION: Adapter molecule that regulates the activation of NF-kappa-B and JNK. Plays a role in the regulation of cell survival and apoptosis. The heterotrimer formed by TRAF1 and TRAF2 is part of a E3 ubiquitin-protein ligase complex that promotes ubiquitination of target proteins, such as MAP3K14. The TRAF1/TRAF2 complex recruits the antiapoptotic E3 protein-ubiquitin ligases BIRC2 and BIRC3 to TNFRSF1B/TNFR2. {ECO:0000269|PubMed:10692572, ECO:0000269|PubMed:16323247, ECO:0000269|PubMed:18429822, ECO:0000269|PubMed:19287455, ECO:0000269|PubMed:19698991, ECO:0000269|PubMed:20385093}.
- Poids moléculaire
- 46.2 kDa
- UniProt
- Q13077
- Pathways
- Signalisation NF-kappaB, Apoptose, Cell-Cell Junction Organization, Asymmetric Protein Localization
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