Taxilin gamma (TXLNG) (AA 1-528) protein (Strep Tag)
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- Antigène Voir toutes Taxilin gamma (TXLNG) Protéines
- Taxilin gamma (TXLNG)
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-528
- Origine
- Humain
- Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Strep Tag
- Application
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Séquence
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MATRVEEAAR GRGGGAEEAT EAGRGGRRRS PRQKFEIGTM EEAGICGLGV KADMLCNSQS NDILQHQGSN CGGTSNKHSL EEDEGSDFIT ENRNLVSPAY CTQESREEIP GGEARTDPPD GQQDSECNRN KEKTLGKEVL LLMQALNTLS TPEEKLAALC KKYADLLEES RSVQKQMKIL QKKQAQIVKE KVHLQSEHSK AILARSKLES LCRELQRHNK TLKEENMQQA REEEERRKEA TAHFQITLNE IQAQLEQHDI HNAKLRQENI ELGEKLKKLI EQYALREEHI DKVFKHKELQ QQLVDAKLQQ TTQLIKEADE KHQREREFLL KEATESRHKY EQMKQQEVQL KQQLSLYMDK FEEFQTTMAK SNELFTTFRQ EMEKMTKKIK KLEKETIIWR TKWENNNKAL LQMAEEKTVR DKEYKALQIK LERLEKLCRA LQTERNELNE KVEVLKEQVS IKAAIKAANR DLATPVMQPC TALDSHKELN TSSKRALGAH LEAEPKSQRS AVQKPPSTGS APAIESVD
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- niveau d'endotoxine
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- Taxilin gamma (TXLNG)
- Autre désignation
- TXLNG (TXLNG Produits)
- Synonymes
- wu:fc50d09 Protein, zgc:136409 Protein, CXHXORF15 Protein, CXorf15 Protein, elrg Protein, fiat Protein, lsr5 Protein, cxorf15 Protein, 4932441K18Rik Protein, Fiat Protein, Lrp Protein, ELRG Protein, FIAT Protein, LSR5 Protein, taxilin gamma Protein, Txlng Protein, txlng Protein, TXLNG Protein
- Sujet
- Gamma-taxilin (Environmental lipopolysaccharide-responding gene protein) (Factor inhibiting ATF4-mediated transcription) (FIAT) (Lipopolysaccharide-specific response protein 5),FUNCTION: May be involved in intracellular vesicle traffic. Inhibits ATF4-mediated transcription, possibly by dimerizing with ATF4 to form inactive dimers that cannot bind DNA. May be involved in regulating bone mass density through an ATF4-dependent pathway. May be involved in cell cycle progression. {ECO:0000269|PubMed:15911876, ECO:0000269|PubMed:18068885}.
- Poids moléculaire
- 60.6 kDa
- UniProt
- Q9NUQ3
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