AXUD1 Protein (AA 1-589) (Strep Tag)
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- Antigène Voir toutes AXUD1 (CSRNP1) Protéines
- AXUD1 (CSRNP1) (Cysteine-serine-Rich Nuclear Protein 1 (CSRNP1))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-589
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Origine
- Humain
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Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette AXUD1 protéine est marqué à la Strep Tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Séquence
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MTGLLKRKFD QLDEDNSSVS SSSSSSGCQS RSCSPSSSVS RAWDSEEEGP WDQMPLPDRD FCGPRSFTPL SILKRARRER PGRVAFDGIT VFYFPRCQGF TSVPSRGGCT LGMALRHSAC RRFSLAEFAQ EQARARHEKL RQRLKEEKLE MLQWKLSAAG VPQAEAGLPP VVDAIDDASV EEDLAVAVAG GRLEEVSFLQ PYPARRRRAL LRASGVRRID REEKRELQAL RQSREDCGCH CDRICDPETC SCSLAGIKCQ MDHTAFPCGC CREGCENPMG RVEFNQARVQ THFIHTLTRL QLEQEAESFR ELEAPAQGSP PSPGEEALVP TFPLAKPPMN NELGDNSCSS DMTDSSTASS SASGTSEAPD CPTHPGLPGP GFQPGVDDDS LARILSFSDS DFGGEEEEEE EGSVGNLDNL SCFHPADIFG TSDPGGLASW THSYSGCSFT SGVLDENANL DASCFLNGGL EGSREGSLPG TSVPPSMDAG RSSSVDLSLS SCDSFELLQA LPDYSLGPHY TSQKVSDSLD NIEAPHFPLP GLSPPGDASS CFLESLMGFS EPAAEALDPF IDSQFEDTVP ASLMEPVPV
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
- Pureté
- > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
- Top Product
- Discover our top product CSRNP1 Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- AXUD1 (CSRNP1) (Cysteine-serine-Rich Nuclear Protein 1 (CSRNP1))
- Autre désignation
- CSRNP1 (CSRNP1 Produits)
- Synonymes
- AXUD1 Protein, CSRNP-1 Protein, FAM130B Protein, TAIP-3 Protein, URAX1 Protein, 4931429D10Rik Protein, Axud1 Protein, taip-3 Protein, CSRNP1 Protein, axud1 Protein, MGC145297 Protein, fb73e11 Protein, wu:fb73e11 Protein, zgc:66340 Protein, cysteine and serine rich nuclear protein 1 Protein, cysteine-serine-rich nuclear protein 1 Protein, cysteine-serine-rich nuclear protein 1 L homeolog Protein, cysteine-serine-rich nuclear protein 1b Protein, CSRNP1 Protein, Csrnp1 Protein, csrnp1 Protein, csrnp1.L Protein, csrnp1b Protein
- Sujet
- Cysteine/serine-rich nuclear protein 1 (CSRNP-1) (Axin-1 up-regulated gene 1 protein) (Protein URAX1) (TGF-beta-induced apoptosis protein 3) (TAIP-3),FUNCTION: Binds to the consensus sequence 5'-AGAGTG-3' and has transcriptional activator activity (By similarity). May have a tumor-suppressor function. May play a role in apoptosis. {ECO:0000250, ECO:0000269|PubMed:11526492}.
- Poids moléculaire
- 63.5 kDa
- UniProt
- Q96S65
- Pathways
- Platelet-derived growth Factor Receptor Signaling
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