ANKZF1 Protein (AA 1-726) (Strep Tag)
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- Antigène Voir toutes ANKZF1 Protéines
- ANKZF1 (Ankyrin Repeat and Zinc Finger Domain Containing 1 (ANKZF1))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-726
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Origine
- Humain
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Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette ANKZF1 protéine est marqué à la Strep Tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Séquence
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MSPAPDAAPA PASISLFDLS ADAPVFQGLS LVSHAPGEAL ARAPRTSCSG SGERESPERK LLQGPMDISE KLFCSTCDQT FQNHQEQREH YKLDWHRFNL KQRLKDKPLL SALDFEKQSS TGDLSSISGS EDSDSASEED LQTLDRERAT FEKLSRPPGF YPHRVLFQNA QGQFLYAYRC VLGPHQDPPE EAELLLQNLQ SRGPRDCVVL MAAAGHFAGA IFQGREVVTH KTFHRYTVRA KRGTAQGLRD ARGGPSHSAG ANLRRYNEAT LYKDVRDLLA GPSWAKALEE AGTILLRAPR SGRSLFFGGK GAPLQRGDPR LWDIPLATRR PTFQELQRVL HKLTTLHVYE EDPREAVRLH SPQTHWKTVR EERKKPTEEE IRKICRDEKE ALGQNEESPK QGSGSEGEDG FQVELELVEL TVGTLDLCES EVLPKRRRRK RNKKEKSRDQ EAGAHRTLLQ QTQEEEPSTQ SSQAVAAPLG PLLDEAKAPG QPELWNALLA ACRAGDVGVL KLQLAPSPAD PRVLSLLSAP LGSGGFTLLH AAAAAGRGSV VRLLLEAGAD PTVQDSRARP PYTVAADKST RNEFRRFMEK NPDAYDYNKA QVPGPLTPEM EARQATRKRE QKAARRQREE QQQRQQEQEE REREEQRRFA ALSDREKRAL AAERRLAAQL GAPTSPIPDS AIVNTRRCWS CGASLQGLTP FHYLDFSFCS TRCLQDHRRQ AGRPSS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- niveau d'endotoxine
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product ANKZF1 Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- ANKZF1 (Ankyrin Repeat and Zinc Finger Domain Containing 1 (ANKZF1))
- Autre désignation
- ANKZF1 (ANKZF1 Produits)
- Synonymes
- ANKZF1 Protein, ZNF744 Protein, RGD1359242 Protein, 1300008P06Rik Protein, 2810025E10Rik Protein, D1Ertd161e Protein, im:7148662 Protein, ankyrin repeat and zinc finger domain containing 1 Protein, ANKZF1 Protein, ankzf1 Protein, Ankzf1 Protein
- Sujet
- TRNA endonuclease ANKZF1 (EC 3.1.-.-) (Ankyrin repeat and zinc finger domain-containing protein 1) (Zinc finger protein 744),FUNCTION: Endonuclease that cleaves polypeptidyl-tRNAs downstream of the ribosome-associated quality control (RQC) pathway to release incompletely synthesized polypeptides for degradation (PubMed:30244831, PubMed:29632312, PubMed:31011209). The RQC pathway disassembles aberrantly stalled translation complexes to recycle or degrade the constituent parts (PubMed:30244831, PubMed:29632312, PubMed:31011209). ANKZF1 acts downstream disassembly of stalled ribosomes and specifically cleaves off the terminal 3'-CCA nucleotides universal to all tRNAs from polypeptidyl-tRNAs, releasing (1) ubiquitinated polypeptides from 60S ribosomal subunit for degradation and (2) cleaved tRNAs (PubMed:31011209). ANKZF1-cleaved tRNAs are then repaired and recycled by ELAC1 and TRNT1 (PubMed:31011209, PubMed:32075755). Also plays a role in the cellular response to hydrogen peroxide and in the maintenance of mitochondrial integrity under conditions of cellular stress (PubMed:28302725). {ECO:0000269|PubMed:28302725, ECO:0000269|PubMed:29632312, ECO:0000269|PubMed:30244831, ECO:0000269|PubMed:31011209, ECO:0000269|PubMed:32075755}.
- Poids moléculaire
- 80.9 kDa
- UniProt
- Q9H8Y5
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