BRPF3 Protein (AA 1-1205) (Strep Tag)
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- Antigène Tous les produits BRPF3
- BRPF3 (Bromodomain and PHD Finger Containing, 3 (BRPF3))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-1205
- Origine
- Humain
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Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette BRPF3 protéine est marqué à la Strep Tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Séquence
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MRKPRRKSRQ NAEGRRSPSP YSLKCSPTRE TLTYAQAQRI VEVDIDGRLH RISIYDPLKI ITEDELTAQD ITECNSNKEN SEQPQFPGKS KKPSSKGKKK ESCSKHASGT SFHLPQPSFR MVDSGIQPEA PPLPAAYYRY IEKPPEDLDA EVEYDMDEED LAWLDMVNEK RRVDGHSLVS ADTFELLVDR LEKESYLESR SSGAQQSLID EDAFCCVCLD DECHNSNVIL FCDICNLAVH QECYGVPYIP EGQWLCRCCL QSPSRPVDCI LCPNKGGAFK QTSDGHWAHV VCAIWIPEVC FANTVFLEPI EGIDNIPPAR WKLTCYICKQ KGLGAAIQCH KVNCYTAFHV TCAQRAGLFM KIEPMRETSL NGTIFTVRKT AYCEAHSPPG AATARRKGDS PRSISETGDE EGLKEGDGEE EEEEEVEEEE QEAQGGVSGS LKGVPKKSKM SLKQKIKKEP EEAGQDTPST LPMLAVPQIP SYRLNKICSG LSFQRKNQFM QRLHNYWLLK RQARNGVPLI RRLHSHLQSQ RNAEQREQDE KTSAVKEELK YWQKLRHDLE RARLLIELIR KREKLKREQV KVQQAAMELE LMPFNVLLRT TLDLLQEKDP AHIFAEPVNL SEVPDYLEFI SKPMDFSTMR RKLESHLYRT LEEFEEDFNL IVTNCMKYNA KDTIFHRAAV RLRDLGGAIL RHARRQAENI GYDPERGTHL PESPKLEDFY RFSWEDVDNI LIPENRAHLS PEVQLKELLE KLDLVSAMRS SGARTRRVRL LRREINALRQ KLAQPPPPQP PSLNKTVSNG ELPAGPQGDA AVLEQALQEE PEDDGDRDDS KLPPPPTLEP TGPAPSLSEQ ESPPEPPTLK PINDSKPPSR FLKPRKVEED ELLEKSPLQL GNEPLQRLLS DNGINRLSLM APDTPAGTPL SGVGRRTSVL FKKAKNGVKL QRSPDRVLEN GEDHGVAGSP ASPASIEEER HSRKRPRSRS CSESEGERSP QQEEETGMTN GFGKHTESGS DSECSLGLSG GLAFEACSGL TPPKRSRGKP ALSRVPFLEG VNGDSDYNGS GRSLLLPFED RGDLEPLELV WAKCRGYPSY PALIIDPKMP REGLLHNGVP IPVPPLDVLK LGEQKQAEAG EKLFLVLFFD NKRTWQWLPR DKVLPLGVED TVDKLKMLEG RKTSIRKSVQ VAYDRAMIHL SRVRGPHSFV TSSYL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
- Pureté
- > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- BRPF3 (Bromodomain and PHD Finger Containing, 3 (BRPF3))
- Autre désignation
- BRPF3 (BRPF3 Produits)
- Synonymes
- BRPF3 Protein, si:dkeyp-46c9.4 Protein, AI413466 Protein, AW493400 Protein, mKIAA1286 Protein, bromodomain and PHD finger containing 3 Protein, bromodomain and PHD finger containing, 3 Protein, bromodomain and PHD finger containing, 3b Protein, bromodomain and PHD finger containing, 3a Protein, BRPF3 Protein, brpf3 Protein, brpf3b Protein, brpf3a Protein, Brpf3 Protein
- Sujet
- Bromodomain and PHD finger-containing protein 3,FUNCTION: Scaffold subunit of various histone acetyltransferase (HAT) complexes, such as the MOZ/MORF and HBO1 complexes, which have a histone H3 acetyltransferase activity (PubMed:16387653, PubMed:26620551, PubMed:26677226). Plays a role in DNA replication initiation by directing KAT7/HBO1 specificity towards histone H3 'Lys-14' acetylation (H3K14ac), thereby facilitating the activation of replication origins (PubMed:26620551). Component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity (PubMed:16387653). {ECO:0000269|PubMed:16387653, ECO:0000269|PubMed:26620551, ECO:0000269|PubMed:26677226}.
- Poids moléculaire
- 135.7 kDa
- UniProt
- Q9ULD4
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