CPEB1 Protein (AA 1-566) (Strep Tag)
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- Antigène Voir toutes CPEB1 Protéines
- CPEB1 (Cytoplasmic Polyadenylation Element Binding Protein 1 (CPEB1))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-566
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Origine
- Humain
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Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette CPEB1 protéine est marqué à la Strep Tag.
- Application
- SDS-PAGE (SDS), Western Blotting (WB), ELISA
- Séquence
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MALSLEEEAG RIKDCWDNQE APALSTCSNA NIFRRINAIL DNSLDFSRVC TTPINRGIHD HLPDFQDSEE TVTSRMLFPT SAQESSRGLP DANDLCLGLQ SLSLTGWDRP WSTQDSDSSA QSSTHSVLSM LHNPLGNVLG KPPLSFLPLD PLGSDLVDKF PAPSVRGSRL DTRPILDSRS SSPSDSDTSG FSSGSDHLSD LISSLRISPP LPFLSLSGGG PRDPLKMGVG SRMDQEQAAL AAVTPSPTSA SKRWPGASVW PSWDLLEAPK DPFSIEREAR LHRQAAAVNE ATCTWSGQLP PRNYKNPIYS CKVFLGGVPW DITEAGLVNT FRVFGSLSVE WPGKDGKHPR CPPKGNMPKG YVYLVFELEK SVRSLLQACS HDPLSPDGLS EYYFKMSSRR MRCKEVQVIP WVLADSNFVR SPSQRLDPSR TVFVGALHGM LNAEALAAIL NDLFGGVVYA GIDTDKHKYP IGSGRVTFNN QRSYLKAVSA AFVEIKTTKF TKKVQIDPYL EDSLCHICSS QPGPFFCRDQ VCFKYFCRSC WHWRHSMEGL RHHSPLMRNQ KNRDSS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
- Pureté
- > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
- Top Product
- Discover our top product CPEB1 Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- CPEB1 (Cytoplasmic Polyadenylation Element Binding Protein 1 (CPEB1))
- Autre désignation
- CPEB1 (CPEB1 Produits)
- Synonymes
- CEBP Protein, CPE-BP1 Protein, CPEB Protein, AU024112 Protein, Cpeb Protein, mCPEB Protein, CPEB1 Protein, cpeb1 Protein, ZOR-1 Protein, cepb1b Protein, fd15c06 Protein, wu:fd15c06 Protein, zorba Protein, cytoplasmic polyadenylation element binding protein 1 Protein, hypothetical protein Protein, cytoplasmic polyadenylation element binding protein 1 L homeolog Protein, cytoplasmic polyadenylation element binding protein 1b Protein, cytoplasmic polyadenylation element binding protein 1 S homeolog Protein, CPEB1 Protein, Cpeb1 Protein, cpeb1 Protein, LOAG_17395 Protein, cpeb1.L Protein, cpeb1b Protein, cpeb1.S Protein
- Sujet
- Cytoplasmic polyadenylation element-binding protein 1 (CPE-BP1) (CPE-binding protein 1) (h-CPEB) (hCPEB-1),FUNCTION: Sequence-specific RNA-binding protein that regulates mRNA cytoplasmic polyadenylation and translation initiation during oocyte maturation, early development and at postsynapse sites of neurons. Binds to the cytoplasmic polyadenylation element (CPE), an uridine-rich sequence element (consensus sequence 5'-UUUUUAU-3') within the mRNA 3'-UTR. RNA binding results in a clear conformational change analogous to the Venus fly trap mechanism (PubMed:24990967). In absence of phosphorylation and in association with TACC3 is also involved as a repressor of translation of CPE-containing mRNA, a repression that is relieved by phosphorylation or degradation (By similarity). Involved in the transport of CPE-containing mRNA to dendrites, those mRNAs may be transported to dendrites in a translationally dormant form and translationally activated at synapses (By similarity). Its interaction with APLP1 promotes local CPE-containing mRNA polyadenylation and translation activation (By similarity). Induces the assembly of stress granules in the absence of stress. Required for cell cycle progression, specifically for prophase entry (PubMed:26398195). {ECO:0000250|UniProtKB:P70166, ECO:0000269|PubMed:15731006, ECO:0000269|PubMed:15966895, ECO:0000269|PubMed:24990967, ECO:0000269|PubMed:26398195}.
- Poids moléculaire
- 62.6 kDa
- UniProt
- Q9BZB8
- Pathways
- Synaptic Membrane
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