NDRG1 Protein (AA 2-394) (His tag)
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- Antigène Voir toutes NDRG1 Protéines
- NDRG1 (N-Myc Downstream Regulated 1 (NDRG1))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 2-394
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Origine
- Humain
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Source
- Cellules d'insectes
- Purification/Conjugué
- Cette NDRG1 protéine est marqué à la His tag.
- Application
- ELISA, SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys)
- Séquence
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SREMQDVDLA EVKPLVEKGE TITGLLQEFD VQEQDIETLH GSVHVTLCGT PKGNRPVILT YHDIGMNHKT CYNPLFNYED MQEITQHFAV CHVDAPGQQD GAASFPAGYM YPSMDQLAEM LPGVLQQFGL KSIIGMGTGA GAYILTRFAL NNPEMVEGLV LINVNPCAEG WMDWAASKIS GWTQALPDMV VSHLFGKEEM QSNVEVVHTY RQHIVNDMNP GNLHLFINAY NSRRDLEIER PMPGTHTVTL QCPALLVVGD SSPAVDAVVE CNSKLDPTKT TLLKMADCGG LPQISQPAKL AEAFKYFVQG MGYMPSASMT RLMRSRTASG SSVTSLDGTR SRSHTSEGTR SRSHTSEGTR SRSHTSEGAH LDITPNSGAA GNSAGPKSME VSC
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Attributs du produit
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human NDRG1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Protein is endotoxin free.
- Classe de qualité
- Crystallography grade
- Top Product
- Discover our top product NDRG1 Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Commentaires
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- NDRG1 (N-Myc Downstream Regulated 1 (NDRG1))
- Autre désignation
- NDRG1 (NDRG1 Produits)
- Synonymes
- CAP43 Protein, CMT4D Protein, DRG-1 Protein, DRG1 Protein, GC4 Protein, HMSNL Protein, NDR1 Protein, NMSL Protein, PROXY1 Protein, RIT42 Protein, RTP Protein, TARG1 Protein, TDD5 Protein, Ndr1 Protein, Ndrl Protein, cap43 Protein, cmt4d Protein, drg1 Protein, gc4 Protein, hmsnl Protein, ndr1 Protein, ndrg1 Protein, nmsl Protein, proxy1 Protein, rit42 Protein, rtp Protein, targ1 Protein, tdd5 Protein, xndrg1 Protein, ndrg4 Protein, cb775 Protein, wu:fb60h02 Protein, zgc:63944 Protein, NDRG1 Protein, NON-RACE SPECIFIC DISEASE RESISTANCE PROTEIN Protein, non race-specific disease resistance 1 Protein, ndrg1-a Protein, ndrg1-b Protein, xNDRG1-A Protein, ndrg1l Protein, zgc:73047 Protein, N-myc downstream regulated 1 Protein, N-myc downstream regulated gene 1 Protein, N-myc downstream regulated 1 S homeolog Protein, N-myc downstream regulated 1a Protein, Late embryogenesis abundant (LEA) hydroxyproline-rich glycoprotein family Protein, N-myc downstream regulated 1 L homeolog Protein, N-myc downstream regulated 1b Protein, NDRG1 Protein, Ndrg1 Protein, ndrg1.S Protein, ndrg1 Protein, ndrg1a Protein, NDR1 Protein, ndrg1.L Protein, ndrg1b Protein
- Sujet
- Stress-responsive protein involved in hormone responses, cell growth, and differentiation. Acts as a tumor suppressor in many cell types. Necessary but not sufficient for p53/TP53-mediated caspase activation and apoptosis. Has a role in cell trafficking, notably of the Schwann cell, and is necessary for the maintenance and development of the peripheral nerve myelin sheath. Required for vesicular recycling of CDH1 and TF. May also function in lipid trafficking. Protects cells from spindle disruption damage. Functions in p53/TP53-dependent mitotic spindle checkpoint. Regulates microtubule dynamics and maintains euploidy. {ECO:0000269|PubMed:15247272, ECO:0000269|PubMed:15377670, ECO:0000269|PubMed:17786215, ECO:0000269|PubMed:9766676}.
- Poids moléculaire
- 43.7 kDa Including tag.
- UniProt
- Q92597
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