PLEKHM1 Protein (AA 1-1056) (Strep Tag)
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- Antigène Voir toutes PLEKHM1 Protéines
- PLEKHM1 (Pleckstrin Homology Domain Containing, Family M (With RUN Domain) Member 1 (PLEKHM1))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-1056
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Origine
- Humain
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Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette PLEKHM1 protéine est marqué à la Strep Tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Séquence
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MLSVVENGLD PQAAIPVIKK KLVGSVKALQ KQYVSLDTVV TSEDGDANTM CSALEAVFIH GLHAKHIRAE AGGKRKKSAH QKPLPQPVFW PLLKAVTHKH IISELEHLTF VNTDVGRCRA WLRLALNDGL MECYLKLLLQ EQARLHEYYQ PTALLRDAEE GEFLLSFLQG LTSLSFELSY KSAILNEWTL TPLALSGLCP LSELDPLSTS GAELQRKESL DSISHSSGSE DIEVHHSGHK IRRNQKLTAS SLSLDTASSS QLSCSLNSDS CLLQENGSKS PDHCEEPMSC DSDLGTANAE DSDRSLQEVL LEFSKAQVNS VPTNGLSQET EIPTPQASLS LHGLNTSTYL HCEAPAEPLP AQAASGTQDG VHVQEPRPQA PSPLDLQQPV ESTSGQQPSS TVSETAREVG QGNGLQKAQA HDGAGLKLVV SSPTSPKNKS WISEDDFYRP SREQPLESAS DHPIASYRGT PGSRPGLHRH FSQEPRKNCS LGALDQACVP SPGRRQAQAA PSQGHKSFRV VHRRQMGLSN PFRGLMKLGT VERRGAMGIW KELFCELSPL EFRLYLSNEE HTCVENCSLL RCESVGPAHS DGRFELVFSG KKLALRASSQ DEAEDWLDRV REALQKVRPQ QEDEWVNVQY PDQPEEPPEA PQGCLSPSDL LSEPAALQGT QFDWSSAQVP EPDAIKESLL YLYMDRTWMP YIFSLSLEAL KCFRIRNNEK MLSDSHGVET IRDILPDTSL GGPSFFKIIT AKAVLKLQAG NAEEAALWRD LVRKVLASYL ETAEEAVTLG GSLDENCQEV LKFATRENGF LLQYLVAIPM EKGLDSQGCF CAGCSRQIGF SFVRPKLCAF SGLYYCDICH QDDASVIPAR IIHNWDLTKR PICRQALKFL TQIRAQPLIN LQMVNASLYE HVERMHLIGR RREQLKLLGD YLGLCRSGAL KELSKRLNHR NYLLESPHRF SVADLQQIAD GVYEGFLKAL IEFASQHVYH CDLCTQRGFI CQICQHHDII FPFEFDTTVR CAECKTVFHQ SCQAVVKKGC PRCARRRKYQ EQNIFA
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- niveau d'endotoxine
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product PLEKHM1 Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- PLEKHM1 (Pleckstrin Homology Domain Containing, Family M (With RUN Domain) Member 1 (PLEKHM1))
- Autre désignation
- PLEKHM1 (PLEKHM1 Produits)
- Synonymes
- AP162 Protein, B2 Protein, OPTB6 Protein, BC038943 Protein, D330036J23Rik Protein, pleckstrin homology and RUN domain containing M1 Protein, pleckstrin homology domain containing, family M (with RUN domain) member 1 Protein, PLEKHM1 Protein, Plekhm1 Protein
- Sujet
- Pleckstrin homology domain-containing family M member 1 (PH domain-containing family M member 1) (162 kDa adapter protein) (AP162),FUNCTION: Acts as a multivalent adapter protein that regulates Rab7-dependent and HOPS complex-dependent fusion events in the endolysosomal system and couples autophagic and the endocytic trafficking pathways. Acts as a dual effector of RAB7A and ARL8B that simultaneously binds these GTPases, bringing about clustering and fusion of late endosomes and lysosomes (PubMed:25498145, PubMed:28325809). Required for late stages of endolysosomal maturation, facilitating both endocytosis-mediated degradation of growth factor receptors and autophagosome clearance. Interaction with Arl8b is a crucial factor in the terminal maturation of autophagosomes and to mediate autophagosome-lysosome fusion (PubMed:25498145). Positively regulates lysosome peripheral distribution and ruffled border formation in osteoclasts (By similarity). May be involved in negative regulation of endocytic transport from early endosome to late endosome/lysosome implicating its association with Rab7 (PubMed:20943950). May have a role in sialyl-lex-mediated transduction of apoptotic signals (PubMed:12820725). Involved in bone resorption (By similarity). {ECO:0000250|UniProtKB:Q5PQS0, ECO:0000250|UniProtKB:Q7TSI1, ECO:0000269|PubMed:12820725, ECO:0000269|PubMed:20943950, ECO:0000269|PubMed:25498145, ECO:0000269|PubMed:28325809}., FUNCTION: (Microbial infection) In case of infection contributes to Salmonella typhimurium pathogenesis by supporting the integrity of the Salmonella-containing vacuole (SCV) probably in concert with the HOPS complex and Rab7. {ECO:0000269|PubMed:25500191}.
- Poids moléculaire
- 117.4 kDa
- UniProt
- Q9Y4G2
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